摘要： 为研究不同浓度葛根素对热应激诱导的LLC-PK1细胞 HSP70 mRNA和蛋白表达的影响，筛选出葛根素诱导HSP70表达的最佳浓度。选取处于对数增长期的猪肾小管上皮细胞，分为37 ℃对照组和42 ℃热应激组，热应激组细胞先在加入不同浓度(0.01~100 μmol/L不等)葛根素的培养基中培养，热应激之后，提取总RNA和总蛋白，用RT-PCR和Western Blot检测 HSP70 mRNA和蛋白的相对表达量。结果显示，42 ℃热应激和不同浓度的葛根素均能显著诱导LLC-PK1细胞的 HSP70 mRNA和蛋白的表达，并且，随葛根素浓度升高，HSP70表达量增加，在各浓度组中，10 μmol/L的葛根素诱导HSP70的表达最显著(P<0.01)。上述结果证明，葛根素能够诱导热应激条件下LLC-PK1细胞 HSP70的表达，并且随葛根素浓度升高，HSP70表达量增加。

关键词: 热休克蛋白, 葛根素, LLC-PK1, HSP70

Abstract: This experiment was to study the effect of puerarin on the thermal stress induced HSP70 expression in pig renal tubular epithelial cell (LLC-PK1), and to selected the optimal concentration of puerarin for inducing HSP70 expression.The LLC-PK1 in its logarithmic growth period was selected and divided into 37 ℃ control group and 42 ℃ heat stress group.The heat stress group of cell was incubated with various concentrations (from 0.01-100 μmol/L) of puerarin before the heat stress treatment, and then the total RNA and protein of the cells were extracted and were detected by RT-PCR and Western Blot, respectively.The results showed that both thermal stress at 42 ℃ and puerarin with various concentrations significantly induced the HSP70 expression of LLC-PK1 (P<0.01), and HSP70 expression increased as the puerarin concentration increased in the culture medium.The relative expression of HSP70 in the heat stress group was the highest in the 10 μmol/L of puerarin group.The above results indicate that puerarin can induce HSP70 expression of LLC-PK1 under heat stress and this expressions increased as the puerarin concentration increased in the culture medium.

Key words: LLC-PK1, Puerarin, Heat stress, HSP70

中图分类号: 

• Q78