摘要： 以多年生日光温室韭菜为试材,以10 mmol/L的硫为总量,研究了无机硫形态及配施对韭菜硝酸盐含量、氮代谢关键酶活性和营养品质的影响。结果表明:NaHSO₃和Na₂S₂O₃配合施用可明显提高硝酸还原酶(NR)、谷氨酸草酰乙酸转氨酶(GOT)、谷氨酸丙酮酸转氨酶(GPT)活性,有效降低硝酸盐含量,其中以7.5 mmol/L NaHSO₃+1.25mmol/L Na₂S₂O₃(S6处理)效果最为明显,降低了35.79%。施硫处理后,韭菜叶片可溶性蛋白质、游离氨基酸、可溶性糖、维生素C含量均有不同程度的增加,且以游离氨基酸增加幅度最大,2.5 mmol/L NaHSO₃+3.75 mmol/L Na₂S₂O₃(S7处理)和5 mmol/L Na₂S₂O₃(S4处理)分别提高了88.89%和83.33%,并且提高了游离氨基酸与可溶性蛋白比值A/P。同时,NaHSO₃和Na₂S₂O₃配合施用也表现出促进韭菜干物质的积累,增加韭菜株高和鲜质量。

关键词: 无机硫形态, 韭菜, 硝酸盐还原同化, 氮代谢关键酶

Abstract: With perennial greenhouse Chinese chive as experimental materials, 10 mmol/L sulfur as total,to study the effects of inorganic sulfur forms on the nitrate content of Chinese chive, nitrogen metabolism and nutrition-al quality.Experimental results show that, the combined application of NaHSO₃ and Na₂S₂O₃ can significantly im-prove the activities of nitrate reductase ( NR), glutamic-oxaloacetic transaminase ( GOT), glutamic-pyruvic transam-inase ( GPT ), and reduce the nitrate content effectively, especially, the effect of 7.5 mmol/L NaHSO₃ +1.25 mmol/L Na₂S₂O₃ is the best,and it can decrease 35.79%.After sulfur treatment,and the content of soluble pro-tein,free amino acids,soluble sugar,Vitamin C in Chinese chive leaves increases in different degrees, especially, free amino acids has the greatest increases.2.5 mmol/L NaHSO₃ +3.75 mmol/L Na₂S₂O₃ ( S7 ) increases 88.89%,and 5 mmol/L Na₂S₂O₃ (S4) increases 83.33% and soluble protein (A1P) increase.Meanwhile,the combined application of NaHSO₃ and Na₂S₂O₃ can also promote the accumulation of dry matter Chinese chive, and increase chives plant height and fresh weight.

Key words: InoRganic sulfuR foRm, Chinese chive, NitRate Reduction and assimilation, NitRogen metabolism activities of key enzymes

中图分类号: 

• S633.3