华北农学报 ›› 2010, Vol. 25 ›› Issue (S1): 17-19. doi: 10.7668/hbnxb.20102.S1.004

所属专题: 畜牧

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中国荷斯坦牛BOLA-DRB上游近端调控区序列分析

王学清, 王昆, 吴占军, 张峰, 李魁英   

  1. 河北省农林科学院粮油作物研究所, 河北省农林科学院奶牛研究中心, 河北 石家庄 050031
  • 收稿日期:2010-03-20 出版日期:2010-12-30
  • 通讯作者: 王昆(1974-),女,河北河间人,副研究员,博士,主要从事动物遗传育种研究
  • 作者简介:王学清(1981-),男,河北平山人,助理研究员,硕士,主要从事动物遗传育种研究
  • 基金资助:
    河北省自然科学基金项目(C2009001308);河北省农林科学院财政项目(2010055008);河北省农林科学院青年基金项目(2009)资助

Nucleotide Sequence of the Upstream Regulatory Region of BOLA-DRB in Chinese Holstein

WANG Xue-qing, WANG Kun, WU Zhan-jun, ZHANG Feng, LI Kui-ying   

  1. Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences,Dairy Cattle Research Center,Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050031, China
  • Received:2010-03-20 Published:2010-12-30

摘要: 通过PCR扩增及DNA序列测定,得到了中国荷斯坦牛BOLA-DRB基因的5'上游近端调控区(URR)序列。比较发现,与人、马以及羊的MHC-DRB基因的5'URR类似,牛MHC-DRB基因5'URR区同样存在与基因表达调控有关的高度保守的W、X、Y、CCAAT及类TATA调控元件,调控元件的组织结构也相同。

关键词: BOLA-DRB 5'URR, MHC-DRB, 调控元件

Abstract: The DNA sequence of the proximal upstream regulatory region(URR) of the Cattle MHC DRB(BO-LA-DRB) genes was amplified using PCR,alignment of the sequence with those of HLA-DRB(GQ345101,X64436,X86151), ELA-DRB (AF344426) and Ovar-DRB(AF344426) showed that: from 5' to 3',it has highly conserve sequence motifs include: W,X,Y,CCAAT and TATA-like regulatory elements,the organization of them was the same too.

Key words: BOLA-DRB 5'URR, MHC-DRB, Regulatory elements

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引用本文

王学清, 王昆, 吴占军, 张峰, 李魁英. 中国荷斯坦牛BOLA-DRB上游近端调控区序列分析[J]. 华北农学报, 2010, 25(S1): 17-19. doi: 10.7668/hbnxb.20102.S1.004.

WANG Xue-qing, WANG Kun, WU Zhan-jun, ZHANG Feng, LI Kui-ying. Nucleotide Sequence of the Upstream Regulatory Region of BOLA-DRB in Chinese Holstein[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S1): 17-19. doi: 10.7668/hbnxb.20102.S1.004.

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