摘要： 为实现抗猪附红细胞体药物的高通量筛选,拟建立小鼠最优感染模型及应用其进行药物敏感性研究。采用三因素三水平的正交试验法,通过对感染率、菌血症峰期及临床症状考察,确定最佳感染方法、感染途径和接种剂量。而后应用感染模型对土霉素、四环素、贝尼尔、青蒿素、咪唑苯脲等10种抗生素和抗原虫药进行药物敏感性测试。方差分析结果显示,以0.1mL剂量尾静脉接种猪EH提纯液,能够使处于免疫抑制状态的昆明小鼠在3d时达到菌血症峰期。药物敏感性测试反映贝尼尔、咪唑苯脲和青蒿素对猪附红细胞体作用最强。因此,用此模型可进行体内抗猪附红细胞体药物的高通量筛选。

关键词: 猪附红细胞体, 小鼠模型, 药物筛选, 药效评价

Abstract: In order to screen drugs efficiently against Eperythrozoonosis suis,an optimal mouse model for EH infection was to be established and applied for studies on drug sensibility. The experiment was based on orthogonal test with 3 factors which each had 3 disparate levels. At first,the optimal infection method,pathway of infection and dosage of inoculation was determined by indexes such as infection rate,peak period of bacteremia and clinical sympton of each experimental group. Then the optimal model was applied to evaluate ten of antibiotics and antiprotozoan agents,containing terramycin,tetracycline,berenil,artemisinin,imidocarb and so on,by the test of drug sensitivity.The result of ANOVA showed that Kunming mice,which were in the state of immunosuppression,went into the peak period of bacteremia immediately within 3 days,when they were inoculated by purified Eperythrozoonosis suis through caudal vein with the dosage of 0. 1 mL. The test of drug sensitivity revealed that berenil,imidocarb and artemisinin had the strongest activity against Eperythrozoonosis suis. Thus,the established model should be used to screen drugs efficiently against Eperythrozoonosis suis.

Key words: Eperythrozoonosis suis, Mouse model, Drug screening, Evaluation of drug effect

中图分类号: 

• S852.62