摘要： 为了解保护地蔬菜丛枝菌根真菌(Arbuscular Mycorrhizal Fungi,AMF)资源及分布特性,调查了6个样点的120份土样中AMF状况.共分离到AMF 属17种,14种鉴定到种,种鉴定到属,分属于球囊霉属(Glomus)、无梗囊霉属(Acaulospora)和巨孢囊霉属(Gigaspora),其中Glomus属分布较为广泛.缩球囊霉(G.constrictum)和幼套球囊霉(G.etunicatum)为优势种,在所有土样中出现的频度较高,说明适应性较强.分析了土壤连作年限、pH值、速效磷含量、全磷含量和有机质含量对丛枝菌根真菌(AMF)物种丰富度和孢子密度的影响,结果表明:保护地蔬菜根围AMF物种丰富度随连作年限的增加而大致呈下降趋势,随pH值(7.0～9.0)的增加而减少,随土壤速效磷含量和土壤全磷含量的增加变化不大;AMF孢子密度随土壤速效磷含量和土壤全磷含量的增加呈明显下降趋势.
关键词:
保护地,
丛枝菌根真菌,
孢子密度,
物种丰富度,
分离频度
Abstract: Diversities of Arbuscular Mycorrhizal Fungi in the soil of vegetables grown in greenhouses were investigated. Three genera(Glomus，Acaulospora and Gigaspora) and 17 species(G. constrictum，G. etunicatum，A. tuberculata， G. mosseae，G. dimorphicum etc) were isolated. Glomus and Acaulospora distributed widely，which frequency were quite high in all of the soil samples. G. constrictum and G. etunicatum were dominant species. The frequency of the former was the highest，which showed it is strong adaptation in this situation. The influence of the replant years，pH value，contents of available P，total P and organic matter on species richness and spore density of AM fungi were analyzed. It was suggested that the spore richness of AM fungi in the soil decreased as the replant years and pH value(7. 0－8. 7) increased. AM fungal spore density decreased as the soil available P content and soil total P content increased.
Key words:
Greenhouse,
Arbuscular mycorrhizal fungi,
Spore density,
Isolation frequency
中图分类号:
杨秀丽, 闫伟. 呼和浩特市保护地蔬菜丛枝菌根真菌初步研究[J]. 华北农学报, 2010, 25(4): 206-208. doi: 10.7668/hbnxb.2010.04.043.
YANG Xiu-li, YAN Wei. A Preliminary Survey of Arbuscular Mycorrhizal Fungi on Greenhouse Vegetables in Huhhot District of Inner Mongolia[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 206-208. doi: 10.7668/hbnxb.2010.04.043.