河北省棉花黄萎菌落叶型和非落叶型菌系初步鉴定

doi:10.7668/hbnxb.2010.04.041

华北农学报 ›› 2010, Vol. 25 ›› Issue (4): 196-200. doi: 10.7668/hbnxb.2010.04.041

所属专题： 棉花；

河北省棉花黄萎菌落叶型和非落叶型菌系初步鉴定

王彦^1,2, 鹿秀云², 郭庆港², 李宝庆², 张乃瑾³, 李社增², 马平², 董金皋¹

1. 河北农业大学植物保护学院, 河北保定 071000;
2. 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 河北保定 071000;
3. 河北省石津灌区管理局, 河北石家庄 050051

收稿日期:2010-06-02 出版日期:2010-08-28
通讯作者: 马平(1964- )，男，江苏南京人，研究员，博士生导师，主要从事棉花病害和植物病害生物防治研究。
作者简介:王彦(1983- ),女,河北邯郸人,硕士,主要从事棉花病害研究.
基金资助:
国家棉花产业技术体系项目;公益性行业(农业)科研专项(nyhyzx07-052)

Molecular Identification of Defoliatiny Pathotype and Non-defoliating Pathotype of Verticillium dahliae from Cotton in Hebei Province

WANG Yan^1,2, LU Xiu-yun², GUO Qing-gang², LI Bao-qing², ZHANG Nai-jin³, LI She-zeng², MA Ping², DONG Jin-gao¹

1. College of Plant Protection, Agricultural University of Hebei, Baoding 071000, China;
2. Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences, IPM Centre of Hebei Province, Baoding 071000, China;
3. Management Bureau of Shijing Irrigation Area in Hebei, Shijiazhuang 050051, China

Received:2010-06-02 Published:2010-08-28

摘要/Abstract

摘要： 利用PCR特异性扩增技术对从采自河北省的228株棉花黄萎菌(Verticillium dahliae)、四川省的2株棉花黄萎菌(菌株Sch-1和Sch-3)、辽宁省的1株黄萎菌(菌株Ly-1)、棉花黄萎菌标准菌株(落叶型标准菌株T9和非落叶型标准菌株V97)和3株棉花枯萎菌(Fusarium oxysporum f.sp.vasinfectum)菌株进行了落叶型和非落叶型菌系的鉴定.结果表明,利用V.dahliae特异性引物DB19/DB22从233株黄萎菌中均可获得00 bp的特异性片段,而3株棉花枯萎菌中不能获得扩增产物.分别利用非落叶型特异性引物INTNDf/INTNDr和落叶型特异性引物INTD2f/INTD2r对233株黄萎菌进行PCR扩增.菌株Sch-1、Sch-3、Ly-1及非落叶型黄萎菌标准菌株V97利用非落叶型特异性引物INTNDf/INTNDr可扩增出1 100 bp的产物;采自河北省的228株黄萎菌和落叶型标准菌株T9利用落叶型特异性引物INTD2f/INTD2r可扩增出40 bp的产物.结果表明,目前河北省主要棉区棉花黄萎病菌以落叶型黄萎菌菌株为主.

关键词: 棉花黄萎菌, 落叶型菌系, 非落叶型菌系, 初步鉴定

Abstract: Two hundred and thirty three strains of V.dahliae and three strains of Fusarium oxysporum f. sp. vasinfectum were isolated from cotton of the major cotton production areas in Hebei，Sichuan and Liaoning provinces. The defoliating and nondefoliating pathotypes of these strains were evaluated by PCR amplification. Three pairs of primers， which are specific for identifying V.dahliae，defoliating and nondefoliating pathotypes，respectively，were used in the PCR analysis. Results showed that the V.dahliae-specific bands were amplified from all the 233 strains of V. dahliae isolates，but not the 3 strains of F.oxysporum f. sp. vasinfectum，by using V. dahliae specific primers DB19 /DB22. PCR amplification with nondefoliating pathotype-specific primers INTNDf / INTNDr revealed that strains Sch-1，Sch-3 from Sichuan province and strain Ly-1 from Liaoning province are nondefoliating type. Defoliating pathotype-specific DNA bands (450 bp) were amplified with primers INTD2f / INTD2r from all the 228 strains of V. dahliae from Hebei province， as well as the defoliating standard strain T9. Our results indicated that defoliating pathotype of V. dahliae was dominant in the cotton growing area of Hebei province.

Key words: Verticillium dahliae, Defoliating pathotype, Non-defoliating pathotype, Molecular identification

中图分类号:

S435.62

王彦, 鹿秀云, 郭庆港, 李宝庆, 张乃瑾, 李社增, 马平, 董金皋. 河北省棉花黄萎菌落叶型和非落叶型菌系初步鉴定[J]. 华北农学报, 2010, 25(4): 196-200. doi: 10.7668/hbnxb.2010.04.041.

WANG Yan, LU Xiu-yun, GUO Qing-gang, LI Bao-qing, ZHANG Nai-jin, LI She-zeng, MA Ping, DONG Jin-gao. Molecular Identification of Defoliatiny Pathotype and Non-defoliating Pathotype of Verticillium dahliae from Cotton in Hebei Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 196-200. doi: 10.7668/hbnxb.2010.04.041.

http://www.hbnxb.net/CN/Y2010/V25/I4/196

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河北省棉花黄萎菌落叶型和非落叶型菌系初步鉴定

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