摘要： 探讨环糊精葡基转移酶的降血糖作用,为进一步研究和产品开发提供依据。通过灌胃和腹腔给药两种方式,研究环糊精葡基转移酶对四氧嘧啶所致糖尿病小鼠血糖的影响,确定其有效作用方式。环糊精葡基转移酶灌胃给药,100,200 mg/kg对血糖无明显影响。环糊精葡基转移酶腹腔给药,不同剂量0.1,1.0,10 mg/kg都可使血糖明显下降,分别下降345.4%,51.2%,43.1%。
关键词:
环糊精葡基转移酶,
血糖,
四氧嘧啶,
糖尿病
Abstract: To investigate the hypoglycemic and antihyperglycemic effects of cyclodextrin glucanotransferase( CGTase)on blood glucose levels in alloxan??induced diabetic rats. Purified CGTase was administrated to alloxan??induced diabeticrats either by oral administration or intraperitoneal injection for consecutive 10 d. Fasting glucose levels in blood samplesof the experimental animals on 2nd, 6th and 10th day and body weights were assayed to assess the bioactivity of CG Tase.The CGTase showed no obvious effects on fasting blood glucose level in alloxan??induced diabetic rats by oral administration neither at a dosage of 100 or 200 mg/ kg per day. On the contrary,when administrated by intraperitoneal injection at various dosages, e. g., 0??1, 1??0 and 10??0 mg/ kg per day, the CGTase decreased significantly the blood glucose levels in the chemically??induced diabet ic rats, and the decreased ratio for each dosage was 45,4%, 51,2%, 43,1%, respectm.
Key words:
Cyclodextrin glucanotransferase,
Blood glucose,
Alloxan,
Diabetes mellitus
中图分类号:
张兴芳, 王荫榆, 郭本恒, 吴正钧, 龚广予. 环糊精葡基转移酶对四氧嘧啶致高血糖模型小鼠血糖的影响[J]. 华北农学报, 2008, 23(S2): 259-262. doi: 10.7668/hbnxb.2008.S2.060.
ZHANG Xing-fang, WANG Yin-yu, GUO Ben-heng, WU Zheng-jun, GONG Guang-yu. Hypoglycemic and Antihyperglycemic Effects of Cyclodextrin Glucanotransferase in Alloxan-induced Diabetic Rats[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S2): 259-262. doi: 10.7668/hbnxb.2008.S2.060.