摘要： 运用PCR技术成功建立了检测产耐热直接相关溶血素(TRH)的副溶血性弧菌的方法,并应用该方法从1株标准株和其他3种不同来源的37株副溶血性弧菌中的13株扩增出0.5 kb的trh基因特异性扩增带;产TRH的副溶血性弧菌中1株标准株,12株分离自腹泻病人排泄物或呕吐物,1株分离自水产动物养殖用水;并且产TRH与尿素酶阳性的副溶血性弧菌菌株之间的具有一一对应的关系,结果认定尿素酶阳性是产TRH的副溶血性弧菌菌株的表现型。

关键词: 副溶血性弧菌, 耐热直接相关溶血素, PCR, 尿素酶

Abstract: In this study,the PCR technology was used to detect the Vibrio parahaemolyticus which could product the TRH,which was one of the major causative agents of Vibrio parahaemolyticus. We found that there were fourteen strains from the 37 Vibrio parahaemolyticus strains can product TRH. In the fourteen strains,one was the type strain,one was isolated from the cultural water,and the other twelve were the clinic strain. The result showed that the strains which produced the TRH can also produced the urase,and the TRH negative ones also the urase negative. So we can judge the Vibrio parahaemolyticus strain which can produce TRH according to the urase test.

Key words: Vibrio parahaemolyticus , TRH, PCR, Urase

中图分类号: 

• TS201.3