摘要： 为研究建立抗生素药物残留的环境生态风险性评估提供方法,建立了鸡排泄物中金霉素残留高效液相色谱分析方法。鸡排泄物经复合提取液均质提取后,调节溶液pH后,离心过滤,以ZORBAX SB-C₁₈色谱柱作为固定相,用0.01 mol/L乙二酸、甲醇、乙腈按77:11.5:11.5体积比混合溶液为流动相,在375 nm波长状态下进行HPLC检测。金霉素浓度在0.1~10.0μg/mL之间,其标准曲线呈线性相关,相关系数r²=0.999 8,在0.5~10.0 mg/kg浓度添加范围内,回收率71.46%~98.01%,日内变异系数0.89%~4.72%,日间变异系数1.94%~7.94%,检出限0.05 mg/kg。

关键词: 金霉素残留, 鸡排泄物, 高效液相色谱-紫外检测器

Abstract: A HPLC method was developed for determination of chlortetracycline (CTC) residues in chicken excrement in this study for the establish of detection method of the antibiotics residues in environment, and offered reference for the environmental risk evaluation system. The chicken excrement samples were extracted with extraction solution and filtrated, then ready to detect. ZORBAX SB-C₁₈ was used for stationary phase, whereas the mobile phase comprised 7710% oxalic acid, 11.5% methanol and 1115% acetonitrile. CTC residues in chicken excrement were detected by VWD at wavelength of 375 nm. The calibration curves for CTC was linear in a concentration range of 011- 1010 Lg/mL (r²= 0.999 8). The recoveries at levels of 0.5, 0.8, 2.0 and 10.0 mg/ kg fortified samples ranged from 71.46% -98.01% with coefficient of variation of intra-assay and inter-assay were 0.89% - 4.72% and 1.94% - 7.94% respectively.The limits of detection were 015 mg/ kg.

Key words: Chlortetracycline residues, Chicken excreta, HPLC-VWD

中图分类号: 

• S859.8