野生花生抗青枯病种质的发掘及分子鉴定

doi:10.7668/hbnxb.2008.03.041

华北农学报 ›› 2008, Vol. 23 ›› Issue (3): 170-175. doi: 10.7668/hbnxb.2008.03.041

所属专题： 油料作物；

野生花生抗青枯病种质的发掘及分子鉴定

陈本银, 姜慧芳, 任小平, 廖伯寿, 黄家权

中国农业科学院油料作物研究所, 湖北, 武汉, 430062

收稿日期:2007-12-28 出版日期:2008-06-28
通讯作者: 姜慧芳(1963-)，女，湖北新洲人，副研究员，博士，主要从事花生品种资源研究工作。
作者简介:陈本银(1981-),女,湖北京山人,硕士,主要从事作物遗传育种研究工作.
基金资助:
国家自然科学基金项目(3027084030571132);国家科技基础条件平台项目(2005DKA21002-13)

Identification and Molecular Traits of Arachis Species with Resistance to Bacterial Wilt

CHEN Ben-yin, JIANG Hui-fang, REN Xiao-ping, LIAO Bo-shou, HUANG Jia-quan

Oil Crops Research Institute, Chinese Academy of Agriculture Sciences, Wuhan 430062, China

Received:2007-12-28 Published:2008-06-28

摘要/Abstract

摘要： 以花生属5个区组的79份野生花生种质为材料,系统鉴定了野生花生对青枯病的抗性反应,从中发掘高抗青枯病的种质15份,含匍匐区组种质3份、直立区组1份、异形花区组1份、花生区组8份、未命名种质2份,抗病材料频率达到19%,高于栽培种花生资源的抗性频率。通过SSR分析表明,在所获得的抗青枯病野生花生材料中,四倍体野生种A.monticola与栽培种花生的亲缘关系最近,其次为花生区组的二倍体野生种A.duranensis和A.chacoense。根据DNA扩增结果,绘制了抗青枯病种质的指纹图谱,明确了其SSR分子特性。

关键词: 花生属, 野生种, 花生青枯病, 抗病种质, SSR分子特性

Abstract: The reaction to bacterial wilt disease of 79 accessions of 18 Arachis species belonging to 5 sections was evaluated in the years from 2004 to 2006.15 genotypes with high resistance were identified including 3 accession of section Procumbentes,1 of section Erectoides,1 of section Heteranthae,8 of section Arachis and 2 of A.sp.The percentage of accessions with resistance was 19% which was much higher than that of cultivated peanut.The relationship and genetic diversity of 15 Arachis accessions with bacterial wilt resistance were investigated based on SSR markers.The tetraploid species A.monticola was most closely related to the cultivated peanut A.hypogaea among the materials involved in the present study.The diploid species A.duranensis and A.chacoense of section Arachis were also closely related to the cultivated peanut.The DNA molecular characters and fingerprints of the 15 accessions were detected based on SSR products.

Key words: Arachis species, Wild species, Peanut bacterial wilt, Resistance, SSR

中图分类号:

S55.2

陈本银, 姜慧芳, 任小平, 廖伯寿, 黄家权. 野生花生抗青枯病种质的发掘及分子鉴定[J]. 华北农学报, 2008, 23(3): 170-175. doi: 10.7668/hbnxb.2008.03.041.

CHEN Ben-yin, JIANG Hui-fang, REN Xiao-ping, LIAO Bo-shou, HUANG Jia-quan. Identification and Molecular Traits of Arachis Species with Resistance to Bacterial Wilt[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(3): 170-175. doi: 10.7668/hbnxb.2008.03.041.

http://www.hbnxb.net/CN/Y2008/V23/I3/170

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