摘要： 采用蒙古口蘑液体深层发酵菌丝体为研究对象,对影响真菌菌丝体凝集素提取的主要因素(浸泡溶液、浸泡时间及不同的硫酸铵分级沉淀浓度)做多因素交叉实验。提取的凝集素生物活性的大小采用凝集素的血凝效价来表示,即能引起血凝现象的凝集素的最高稀释倍数2″表示。结果表明:浸泡溶液、浸泡时间及不同的硫酸铵分级沉淀浓度都显著影响提取的凝集素相对含量。并获得最佳提取工艺为:浸泡溶液0.01 mol/L PBS(pH 值7.4),浸泡时间48h 和分级沉淀浓度20%～75%。
关键词:
蒙古口蘑,
凝集素,
凝血活性
Abstract: Using the mycelium of Tricholoma mongolicuni Imai which produced by submerged fermentationas materials,the mufti-factors cross-over experiments was designed to discuss the factors (different soakingsolution,soaking time and ammonium sulphate precipitation concentration gradient)which influence the extraction of lectins.The biological activities of lectins is expressed by the hemag glutinating maximum multipleof the dilution.The results showed:in the most effective extraction teehnology,0.0lmo1/L PBS (pH7.4) wasselected assoaking solution;48 hours as soaking durations and 20%-75% as ammonium sulphate precipitationconcentration gradient.
Key words:
Tricholonm mongolicum,
Lectin,
EIemagglutinating activity
中图分类号:
姚庆智, 王飞, 渠志臻, 闫伟. 蒙古口蘑液体深层发酵菌丝体凝集素提取工艺研究[J]. 华北农学报, 2007, 22(S3): 79-82. doi: 10.7668/hbnxb.2007.S3.019.
YAO Qing-zhi, WANG Fei, QU Zhi-zheng, YAN Wei. Study on the Extraction Technology of Lectins from the Mycelium Produced by Submerged Fermentation of Tricholoma Mongolicum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(S3): 79-82. doi: 10.7668/hbnxb.2007.S3.019.