摘要： 利用AFLP指纹图谱分析技术对57个西瓜材料进行了基因组DNA多态性分析。结果表明,18对AFLP引物共产生709条DNA带,平均每个引物组合扩增出39.4条带,其中440条为多态性带,占总带数的61.4%;根据DNA谱带计算品种间遗传相似系数,其变化范围在0.24~0.99之间,平均相似系数为0.70,说明西瓜品种间遗传基础狭窄。用NTSYS软件进行聚类分析,57个西瓜品种被划分为2个类群。其中类群1包括1个野生西瓜,类群2均为栽培种。在类群2中引进的资源聚在一起,杂交种和双亲或其一聚在一起。此外,本研究表明,AFLP指纹分析技术具有很高的分辨率,能够揭示西瓜品种间的差异,是鉴定西瓜品种的有力工具。

关键词: 西瓜, 品种, 遗传多样性, 亲缘关系, AFLP分析

Abstract: 57 watermelon materials were analyzed using AFLP.The results showed that 709 AFLP bands were ampli??fied using 18 primer pairs, among them, 440( 61. 4%) bands were polymorphic. Based on DNA bands, the similarity coefficient were calculated and their average value was 0. 70, ranging from 0. 24 to 099,which indicated that relationship wasclose and their genetic base was narrow among materials of watermelons.When all of the polymorphic bands were analyzedby cluster analysis, 57 watermelon materials were classified into 2 groups. Group included 1 wild watermelon material, andgroup, 56 watermelon materials fell into it. Among the group introduced watermelon materials were clustered together aswell as the F1 and parent materials. In addition, this study demonstrates that AFLP fingerprint ing has higher resolution andcan reveal the difference of cult ivars, so it is an efficient tool.

Key words: Watermelon, Cultivar, Genetic diversity, Relationship, AFLP analysis

中图分类号: 

• S651