一株腐殖质还原功能菌的分离筛选与鉴定

doi:10.3969/j.issn.1000-7091.2013.04.033

华北农学报 ›› 2013, Vol. 28 ›› Issue (4): 179-183. doi: 10.3969/j.issn.1000-7091.2013.04.033

一株腐殖质还原功能菌的分离筛选与鉴定

陈楠^1,2,3, 邓晓^1,3, 邹雨坤^1,3, 武春媛^1,3, 李勤奋^1,3

1. 中国热带农业科学院环境与植物保护研究所, 海南海口 571101;
2. 海南大学环境与植物保护学院, 海南海口 570228;
3. 农业部儋州农业环境科学观测实验站, 海南儋州 571737

收稿日期:2013-05-03 出版日期:2013-08-28
通讯作者: 李勤奋 (1974－ )，女，内蒙古鄂尔多斯人，副研究员，博士，主要从事生态农业技术研究与应用武春媛(1981－ )，女，内蒙古巴彦淖尔人，助理研究员，博士，主要从事农药环境行为研究
作者简介:陈楠 (1987－ )，女，内蒙古呼和浩特人，在读硕士，主要从事环境微生物学研究
基金资助:
国家自然科学基金资助项目(41101477);国家科技支撑计划子课题项目(2012BAC18B04-5);海南省重点科技项目(ZDXM20100021)

Isolation and Identification of a Humic Substances Bacterial Strain

CHEN Nan^1,2,3, DENG Xiao^1,3, ZOU Yu-kun^1,3, WU Chun-yuan^1,3, LI Qin-fen^1,3

1. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science, Haikou 571101, China;
2. College of Environment and Plant Protection of Hainan University, Haikou 570228, China;
3. Danzhou Scientific Observing and Experimental Station of Agro-Environment, Ministry of Agricultural, Danzhou 571737, China

Received:2013-05-03 Published:2013-08-28

摘要/Abstract

摘要： 腐殖质呼吸是一种新型微生物能量代谢方式,菌种资源研究成为腐殖质呼吸的研究重点。利用厌氧富集分离方法,从木薯堆肥土壤中获得1株具有腐殖质还原特性的兼性厌氧菌株,编号为HN02。HN02为革兰氏阳性菌,细胞为短杆状,菌落呈黄色,表面光滑,边缘整齐;最适生长盐度为1%,最适生长温度为30℃,最适生长pH值7.5～8.5;可利用麦芽糖作为碳源,明胶液化和硝酸盐还原为阴性;16S rDNA基因序列分析结果表明:菌株HN02与天花板节杆菌亲缘关系最近,同源性达99.4%;HN02能在以葡萄糖作为电子供体,以AQDS为唯一电子受体的厌氧环境下将AQDS还原为AH2QDS,还原率达40%。菌株HN02是1株具有腐殖质还原特性的天花板节杆菌,首次报道该菌属菌株具有腐殖质还原特性,为完善腐殖质还原特性的微生物系统提供理论基础。

关键词: 腐殖质, 还原菌, 节杆菌属

Abstract: The humus respiration is a new way of microbial energy metabolism．And the research about strains resources becomes the focus point．A facultative anaerobic humus- reducing bacterium，designated HN02，was isola- ted from cassava dregs composting．Strain HN02 is a gram- positive，short rod- shaped，gelatin hydrolysis and nitrate reduction- negative bacterium．Optimal growth salt was 1% (NaCl); optimal growth temperature was 30 ℃ and opti- mal pH was at 7． 5－ 8． 5． Analysis of 16S rDNA of HN02 indicated that the closest phylogenetic relative among the valid species was Arthrobacter tecti，with 99． 4% 16S rDNA similarity．Strain HN02 can utilize AQDS via anaerobic enrichment procedure with glucose as the electron donor and AQDS as the sole terminal electron acceptor．The re- duction ratio of AQDS was 40%．Thus，the humus- reducing of Arthrobacter tecti HN02 is here reported for the first time and it can provides the theoretical basis for microbial systems with characteristics of humus reduction．

Key words: Humic substances- reducing, Bacterium, Arthrobacter

中图分类号:

S432.4

陈楠, 邓晓, 邹雨坤, 武春媛, 李勤奋. 一株腐殖质还原功能菌的分离筛选与鉴定[J]. 华北农学报, 2013, 28(4): 179-183. doi: 10.3969/j.issn.1000-7091.2013.04.033.

CHEN Nan, DENG Xiao, ZOU Yu-kun, WU Chun-yuan, LI Qin-fen. Isolation and Identification of a Humic Substances Bacterial Strain[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(4): 179-183. doi: 10.3969/j.issn.1000-7091.2013.04.033.

http://www.hbnxb.net/CN/Y2013/V28/I4/179

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