华北农学报 ›› 2012, Vol. 27 ›› Issue (3): 173-176. doi: 10.3969/j.issn.1000-7091.2012.03.033

所属专题: 烟草 植物保护

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3种农药对烤烟旺长期叶绿素含量及相关酶活性的影响

刘鹏飞1, 段宾宾1, 赵铭钦1, 李元实2, 韩富根1   

  1. 1. 河南农业大学 烟草学院/国家烟草栽培生理生化基地, 河南郑州 450002;
    2. 吉林烟草工业有限责任公司, 吉林延吉 133001
  • 收稿日期:2012-03-12 出版日期:2012-06-28
  • 通讯作者: 韩富根(1953-), 男, 河南鄢陵人, 教授, 主要从事烟草栽培与烟草化学方面的教学与科研工作。
  • 作者简介:刘鹏飞(1981-), 男, 河南安阳人, 讲师, 博士, 主要从事烟草化学与烟用香料方面的教学与科研工作。
  • 基金资助:
    吉林烟草工业有限责任公司重大科技攻关项目(JY2006012)

Impact of Three Kinds of Pesticides on Period Chlorophyll Content and Related Enzyme Activities in Tobacco Rapid Growth

LIU Peng-fei1, DUAN Bin-bin1, ZHAO Ming-qin1, LI Yuan-shi2, HAN Fu-gen1   

  1. 1. College of Tobacco Science, Henan Agricultural University/ National Tobacco Cultivation & Physiology & Biochemistry Research Center, Zhengzhou 450002, China;
    2. Jilin Tobacco Industrial CO. , LTD, Yanji, Jilin 133001, China
  • Received:2012-03-12 Published:2012-06-28

摘要: 为了研究大田中常用3种化学农药对烤烟旺长期生理活性的影响,选用3种不同农药(氧化乐果乳油、乙酰甲胺磷乳油、吡虫啉可湿性粉剂),测定其在烟苗旺长期对烤烟叶片的叶绿素含量和吲哚乙酸氧化酶(IAAO)、硝酸还原酶(NR)、转化酶(InV)、苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)等5种酶活性的影响。结果表明,喷施3种农药均不同程度地降低了叶片中叶绿素含量、NR和InV活性,并随着烟草生育期的推进,上述指标均有所提高;而IAAO,PAL和PPO活性在施药后明显增加,随着烟草生育期的推进回落明显。这3种农药对烟草叶片中不同的生理活性指标有着不同程度的影响。

关键词: 烤烟, 叶片, 叶绿素, 酶, 活性

Abstract: The purpose of this study was to research the impact of physiological activity with application of 3?kinds of pesticides. The impact of chemical pesticides on tobacco leaf's physiological activity including chlorophyll?content,IAAO,NR,InV,PAL and PPO activities were researched with application of 3 kinds of chemical pesticides?(omethoate EC,acephate EC,imidacloprid WP)were used in these experiment assays. The results showed that,the?content of chlorophyll,activities of NR and InV were reduced after spraying this three kinds of pesticides. With the?promotion of tobacco plants growing period,these indicators rebounded significantly;On the contrary,the activities of?IAAO,PAL and PPO were significantly increased after application. With the promotion of tobacco plants growing period,these indicators reduced significantly. These three kinds of pesticide on tobacco leaves with different physiological parameters have different impact.

Key words: Flue-cured tobacco, Leaf, Chlorophyll, Enzyme, Activity

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引用本文

刘鹏飞, 段宾宾, 赵铭钦, 李元实, 韩富根. 3种农药对烤烟旺长期叶绿素含量及相关酶活性的影响[J]. 华北农学报, 2012, 27(3): 173-176. doi: 10.3969/j.issn.1000-7091.2012.03.033.

LIU Peng-fei, DUAN Bin-bin, ZHAO Ming-qin, LI Yuan-shi, HAN Fu-gen. Impact of Three Kinds of Pesticides on Period Chlorophyll Content and Related Enzyme Activities in Tobacco Rapid Growth[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 173-176. doi: 10.3969/j.issn.1000-7091.2012.03.033.

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