摘要： 为探讨西芹鲜根及根际区物化感物质成分,利用柱层析法及GC-MS对西芹鲜根及根际区物不同浸提液二次层析后化感效应最佳流分进行分离鉴定。结果表明,西芹鲜根与根际区物乙醇浸提液二次层析后化感效应最佳流分的主要化感成分均为4-二甲氨基-2-甲基-1-苯基-丁-2-醇、8-十八烯酸甲酯、棕榈酸甲酯、十八烷二烯酸甲酯;西芹鲜根与根际区物丙酮浸提液二次层析后化感效应最佳流分的主要化感成分为2-丙烯酸十五烷基酯;西芹鲜根与根际区物水浸提液二次层析后化感效应最佳流分的主要化感成分为十六烷基二甲基叔胺、14-甲基十五烷酸甲酯、六甲基环三硅氧烷、(E)-9-十八烯酸甲酯、蓖麻油酸甲酯、16-甲基十七烷酸甲酯。
关键词:
西芹,
鲜根,
根际区物,
化感物质,
成分鉴定
Abstract: In order to investigate the allelochemicals in the root and the rhizosperic soil of parsley，the column?chromatography and GC-MS were used to study the best allelopathy fraction of different extracts in the root and the?rhizosperic soil of parsley after secondary separation. The results showed the main allelochemicals of the best allelopathy fraction of the ethanol extract in the root and the rhizosperic soil of parsley after secondary separation were 4Dimethylamino-2-methyl-1-phenyl-butan-2-ol，8-Octadecenoic acid，methyl ester，Hexadecanoic acid，methyl ester, Octadecadienoic acid，methyl ester;the main allelochemicals of the best allelopathy fraction of the acetone extract in?the root and the rhizosperic soil of parsley after secondary separation were 2-Propenoic acid，pentadecyl ester;the?main allelochemicals of the best allelopathy fraction of the aqueous extract in the root and the rhizosperic soil of?parsley after secondary separation were 1-Hexadecanamine，N，N-dimethyl-，Pentadecanoic acid，14-methyl-，methyl?ester，Cyclotrisiloxane，hexamethyl-，9-Octadecenoic acid，methyl ester，(E)-，Methyl ricinoleate，Heptadecanoic?acid，16-methyl-，methyl ester.
Key words:
Parsley,
Root ,
Rhizosperic soil,
Allelochemicals,
Identification
中图分类号:
陈磊, 云兴福. 西芹鲜根及根际区物化感物质成分鉴定[J]. 华北农学报, 2012, 27(2): 157-164. doi: 10.3969/j.issn.1000-7091.2012.02.030.
CHEN Lei, YUN Xing-fu. Allelochemicals Identification in the Root and the Rhizosperic Soil of Parsley[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 157-164. doi: 10.3969/j.issn.1000-7091.2012.02.030.