摘要： 在伪狂犬病病毒种特异性单克隆抗体的基础上建立了检测该病毒抗原的单抗夹心LAB-ELISA方法。结果表明，该方法不与其他常见病原体产生交叉反应，抗原的最低检出含量为8.9μg/mL。检测时最佳采样部位是猪脑及扁桃体。对人工感染兔、自然感染猪以及临床可疑病猪的检出率分别为75%，75%及72.7%，因此本方法是一种具有良好特异性及较好敏感性的诊断方法。
关键词:
猪伪狂犬病病毒,
LAB-ELISA,
检测：诊断
Abstract: A sandwich LAB-ELISA was developed to detect Pseudorabies virus (PrV) on the basis of species specific monoclonal antibodies in this study.The results showed that there was no cross reactions between the PrV and other common pathogens.The minimum concentration of the antigen that could be detected with this assay was 8.9μg/mL.The most suitable organ to get samples was brain or tonsil.The positive rates to artificially infected rabbits,naturally infected swine and clinical dubious swine were 75%,75% and 72.7%.Therefore this assay was a suitable method with good specificity and sensitivity in the diagnosis of Pseudorabies.
Key words:
Pseudorabies virus,
LAB-ELISA,
Detection,
Diagnosis
中图分类号:
苗得园, 杨兵, 张培君, 龚玉梅, 李永清, 李富强, 付磊, 高配亮. 单抗夹心LAB-ELISA检测猪伪狂犬病病毒的研究[J]. 华北农学报, 2001, 16(1): 127-131. doi: 10.3321/j.issn:1000-7091.2001.01.024.
MIAO Deyuan, YANG Bing, LI Fuqiang, ZHANG Peijun, GONG Yumei, LI Yongqing, FU Lei, GAO Peiliang. Study of Monoclonal Antibodies Sandwich LAB-ELISA Detecti ng Pseudorabies Virus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2001, 16(1): 127-131. doi: 10.3321/j.issn:1000-7091.2001.01.024.