摘要： 进行了禽脑脊髓炎病毒L2Z株在BGM 70传代细胞系上培养特性的研究。该毒株在细胞上盲传3代后能产生明显的细胞病变，并且传代过程中细胞病变稳定地出现。利用琼脂扩散试验、免疫荧光试验、免疫酶试验及负染电镜观察研究表明，L2Z株接种BGM 70细胞具有良好的增殖特性，并且病毒是在细胞浆中进行复制.该研究报道禽脑脊髓炎病毒能成功地在传代细胞系上培养增殖。
关键词:
禽脑脊髓炎病毒,
BGM-70传代细胞系,
培养特性,
细胞病变
Abstract: A study on the culture characteristics of avian encephalomyelitis virus (AEV) strain L 2Z in continuous cell line BGM 70 was carried out. The strain blindly passaged to the third generation in BGM 70 cell line caused pronounced cytopathic effects (CPE) , and produced consistently CPE from the third passage. These results from the agar gel diffusion precipitin (AGP) , indirect immunofluorescence antibody (IFA) , indirect immunoenzyme assay (IIEA) and negatively stained electron microscope (NSEM) techniques showed that the strain L 2Z adapted to BGM 70 cell line had good multiplication characteristics, and its replicated area was cytoplasma. This paper is the first report on the successful cultivation and multiplication of avian encephalomyelitis virus in continuous cell line.
Key words:
Avian encephalomyelitis virus(AEV),
Continuous cell line BGM 70,
Cultrue characteristics,
Cytopathic effects(CPE)
刘爵, 张杰, 刘有昌, 张方亮, 周蛟. 禽脑脊髓炎病毒在BGM-70传代细胞系上培养特性的初步研究[J]. 华北农学报, 1999, 14(3): 136-140. doi: 10.3321/j.issn:1000-7091.1999.03.028.
Liu Jue, Zhang Jie, Liu Youchang, Zhang Fangliang, Zhou Jiao. A Preliminary Study on the Culture Characteristics of Avian Encepholomyelitis Virus in Continuous Cell Line BGM-70[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1999, 14(3): 136-140. doi: 10.3321/j.issn:1000-7091.1999.03.028.