为解决露天煤矿排土场复垦土壤植被恢复困难的问题，探索有效地改良土壤的措施，采用盆栽试验方法测定排土场土壤中菌剂与肥料配施对紫花苜蓿的生长发育的影响，进而探讨菌根剂与肥料的配施对矿区土壤的改良作用。结果表明：菌根剂与肥料配合施用能显著提高紫花苜蓿的株高、生物量、叶绿素、蛋白质、可溶性糖的含量以及抗氧化酶同工酶活性等，土壤中速效磷、速效氮、有机质、全氮、全磷含量以及土壤微生物数量也都有明显提高。其中尤以有机肥+无机肥+菌剂配施效果最为显著，与对照相比，株高、鲜质量、干质量分别增加47%，277%，280%，叶绿素、蛋白质、可溶性糖含量分别增加36%，183%，59%，过氧化氢酶、过氧化物酶、超氧化物歧化酶活性分别增加281%，1250%，15%，土壤速效N、速效P含量分别增加130%，217%，有机质含量增加76%，全N、全P含量分别增加102%，160%，土壤细菌、放线菌、真菌数量分别增加103%，68%，20%。总之，有机肥+无机肥+菌剂配施显著促进了紫花苜蓿的生长，提高了排土场土壤的养分含量，是矿区复垦土壤植被恢复中比较好的组合方式。
Opencast coal mining leaves large areas of refuse dump,because of the environment pollution,soil erosion、nutrient-poor of soil and biodiversity loss,vegetation reclamation in these areas is difficult,so explore the measures for soil improvement is important to practice.In this paper,the effect of combined application of microbial inoculum and fertilizer on growth of alfalfa in refuse dump through pot culture experiment,and then explore the effect of combined application of microbial inoculum and fertilizer on improvement of mine soil.The results showed that combined application of microbial inoculum and fertilizer significantly increased the alfalfa's biomass,plant height,the content of chlorophyll,protein,soluble sugar and activity of antioxidant enzyme isozyme,nutrient content of reclaimed soil was also significantly increased.The most effective treatments was organic fertilizer+inorganic fertilizer+microbial inoculum applied,compared with the control,the increasing rates of plant height,fresh weight and dry weight reached 47%,277% and 280%,the increasing rates of chlorophyll content,protein content and soluble sugar content reached 36%,183% and 59%,the increased rates of hydrogen peroxide activity,peroxidase activity and superoxide dismutase activity reached 281%,1250% and 15%,the increased rates of soil available N,P,organic matter,total N and P reached 130%,217%,76%,102% and 160%,the increased rates of bacteria,actinomycetes and fungi reached 103%,68% and 20%.In general,combined application of microbial inoculum,inorganic fertilizer and organic fertilizer significantly promoted the growth of alfalfa and improved the fertility of dump soil,was a good combination of vegetation reclamation in mine wasteland soil.
[1] 吴景龙.内蒙古煤炭资源开发利用现状及对策研究[J].煤炭工程,2008(2):101-102.
[2] Peat H,Fitter A H.The distributuon of arbuscular mycorrhizas in the British flora[J].New Phytologist,1993,125:845-854.
[3] Fitter A H.The role and ecological significane of vesicular-arbuscular mycorrhizas in themperate ecosystems[J].Agriculture,Ecosystems and Environment,1989,29:137-151.
[4] 毕银丽,吴福勇,武玉坤.丛枝菌根在煤矿区生态重建中的应用[J].生态学报,2005,25(8):2068-2073.
[5] Akhilesh K,Nivedita,Upadhyay R S.VA mycorrhizae and revegetation of coal mine spoils:A review[J].Tropical Ecology,1999,40(1):1-10.
[6] Barea J M,Toro M,Orozco M O, et al. The application of isotopic(32P and 15N) dilution techniques to evaluate the interactive effect of phosphate-solubilizing rhizobacteria,mycorrhizal fungi and rhizobium to improve the agronomic efficiency of rock phosphate for legume crops[J].Nutrient Cycling in Agroecosystems,2002,63(1):35-42.
[7] 吴建峰,林先贵.我国微生物肥料研究现状及发展趋势[J].土壤,2002,34(2):68-72.
[8] 包玉英,闫伟,张美庆.内蒙古草原常见植物根围AM真菌[J].菌物学报,2007,26(1):51-58.
[9] 范文斌,李长青,刘永斌.在内蒙古地区开展牛粪厌氧发酵技术生产沼气的探索[J].畜牧与饲料科学,2009,30(1):127-128.
[10] 袁立,王占哲,刘春龙.国内外牛粪生物质资源利用的现状与趋势[J].中国奶牛,2011(5):3-9.
[11] 宋秀杰.我国有机肥利用现状及合理利用的技术措施[J].农村生态环境,1997,13(2):57-60.
[12] 高菊生,徐明岗,王伯仁,等.长期有机无机肥配施对土壤肥力及水稻产量的影响[J].中国农学通报,2005,21(8):211-214,259.
[13] 梁利宝,洪坚平,谢英荷.不同培肥处理对采煤塌陷土地复垦不同年限土壤熟化的影响[J].水土保持学报,2010,24(3):140-144.
[14] 马从安,才庆祥.哈尔乌素露天煤矿排土场土壤肥力综合评价[J].露天采矿技术,2010(3):56-58.
[15] 李建华,郜春花,卢朝东,等.菌剂与肥料配施对矿区复垦土壤白三叶草生长的影响[J].中国生态农业学报,2011,19(2):280-284.
[16] 张治安,张美善,蔚荣海.植物生理学实验指导[M].北京:中国农业技术出版社,2004.
[17] 鲍士旦.土壤农化分析[M].北京:中国农业出版社,2000.
[18] 程量,林良斌.作物耐旱性生理生化指标研究进展[J].中国农学通报,2014,30(3):27-31.
[19] Chen Y W,Shao G H,Chang R Z.The effect of salt stress on superoxide dismutase in various organelles from cotyledon of soybean seeding[J].Acta Agronomica Sinica,1997,23(2):214-217.
[20] Johansen A.Hyphal transport of 15 N-labelled nitrogen by a vesicular-arbuscular mycorrhizal fungus and it s effect on depletion of inorganic soil[J].New Phytol,1992,122:281-285.
[21] Ezawa T,Smith S E,Smith F A.P metabolism and transport in AM fungi[J].Plant and Soil,2002,244(1/2):221-230.
[22] 赵征宇,孙永红,赵明,等.有机无机肥配施对土壤氮素转化和番茄产量品质的影响[J].华北农学报,2013,28(1):208-212.
[23] Dere A L,Stehouwer R C,McDonald K E.Nutrient leaching and switchgrass growth in mine soil columns amended with poultry manure[J].Soil Science,2011,176(2):84-90.
[24] 周爽,白瑞琴,张海勃.重金属Cd 及施肥对马铃薯抗氧化酶系统的影响[J].干旱区资源与环境,2012,26(9):88-92.
[25] Moreira F M S,de Carvalho T S,Siqueira J O.Effect of fertilizers,lime,and inoculation with rhizobia and mycorrhizal fungi on the growth of four leguminous tree species in a low-fertility soil[J].Biol Fertil Soils,2010,46:771-779.
[26] 鲁叶江,李树志,张春娜.东部平原矿区不同培肥处理对复垦土壤特性的影响[J].中国农学通报,2012,28(5):221-225.
[27] 唐继伟,林治安,许建新,等.有机肥与无机肥在提高土壤肥力中的作用[J].中国土壤与肥料,2006(3):44-47.
[28] Wang L P,Qian K M,He S L, et al.Fertilizing reclamation of arbuscular mycorrhizal fungi on coal mine complex substrate[J].Procedia Earth and Planetary Science,2009,1(1):1101-1106.
