论文

GmNHX1LcVP1双价基因植物表达载体构建与转基因棉花的获得

  • 冯雪 ,
  • 贾永红 ,
  • 郭红珍 ,
  • 张一名 ,
  • 赵学良 ,
  • 孙艳香
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  • 廊坊师范学院 遗传与育种研究所, 河北 廊坊 065000
冯雪(1978-),女,河北霸州人,讲师,硕士,主要从事植物分子生物学与基因工程教学和研究。

收稿日期: 2014-03-12

  网络出版日期: 2014-09-20

基金资助

河北省自然科学基金项目(C2010001804);河北省高等学校科学技术研究项目(20070318);廊坊师范学院博士基金项目(LSZB200903);廊坊师范学院自然科学重点项目(LSZZ201102)

Construction of Plant Expression Vector with GmNHX1 and LcVP1 Dual-gene and Obtaining Transgenic Cotton

  • FENG Xue ,
  • JIA Yong-hong ,
  • GUO Hong-zhen ,
  • ZHANG Yi-ming ,
  • ZHAO Xue-liang ,
  • SUN Yan-xiang
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  • Institute of Genetics and Breeding, Langfang Normal University, Langfang 065000, China

Received date: 2014-03-12

  Online published: 2014-09-20

摘要

应用DNA重组技术将编码大豆液泡膜Na+/H+反向转运蛋白GmNHX1和百脉根液泡膜H+-PPase LcVP1的开放阅读框片段同时构建在pCambia1301载体中,形成p1301-GmNHX1-LcVP1 双价耐盐基因植物表达载体。以Coker 312棉花品种的胚性愈伤组织为外植体,采用根癌农杆菌介导法将其转入棉花中,并利用潮霉素筛选获得抗性植株。对抗性植株的T1幼苗进行分子和生理指标检测,表明外源基因已成功整合到棉花基因组中,并不同程度地提高了转基因株系耐盐能力。

本文引用格式

冯雪 , 贾永红 , 郭红珍 , 张一名 , 赵学良 , 孙艳香 . 含GmNHX1LcVP1双价基因植物表达载体构建与转基因棉花的获得[J]. 华北农学报, 2014 , 29(4) : 56 -62 . DOI: 10.7668/hbnxb.2014.04.010

Abstract

By use of DNA recombination method,a vector,p1301-GmNHX1-LcVP1, harboring two open reading frames of soybean tonoplast Na+/H+ antiporter GmNHX1 and Lotus corniculatus H+-PPase LcVP1,was constructed through sequential restriction digests and ligations recombination.The two genes driven by CaMV 35S promoters were successfully transferred into cotton cultivars Coker 312 via Agrobacterium with hygromycin selection system.The molecular and physiological analysises of T1 generation indicated that genes were integrated into the cotton genome and enhanced transgenic cotton salt tolerance.

参考文献

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