论文

甘蓝硫代葡萄糖苷总量性状的QTL定位及分析

  • 田多成 ,
  • 何洪巨 ,
  • 严慧玲 ,
  • 颉建明 ,
  • 康俊根
展开
  • 1. 甘肃农业大学 农学院, 甘肃 兰州 730070;
    2. 北京市农林科学院 蔬菜研究中心, 农业部华北地区园艺作物 生物学与种质创制重点实验室, 北京 100097;
    3. 河北省农林科学院 经济作物研究所, 河北 石家庄 050051
田多成(1987-),男,甘肃礼县人,在读硕士,主要从事蔬菜分子育种研究。

收稿日期: 2014-03-12

  网络出版日期: 2014-09-19

基金资助

国家自然科学基金项目(31171958);“十二五”国家科技支撑计划项目(2012BAD02B01;2012BAD50G01);“863”项目(2012AA100105;2012AA020103)

Mapping QTL and Analysis on Glucosinolates Total Content in Cabbage

  • TIAN Duo-cheng ,
  • HE Hong-ju ,
  • YAN Hui-ling ,
  • XIE Jian-ming ,
  • KANG Jun-gen
Expand
  • 1. College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China;
    2. Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China), Ministry of Agriculture, Beijing 100097, China;
    3. Institute of Economic Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China

Received date: 2014-03-12

  Online published: 2014-09-19

摘要

采用HPLC方法分析了作图群体双亲及F2 83个单株的硫代葡萄糖苷总含量,在已构建的高密度甘蓝遗传图谱的基础上,运用MapQTL 4.0软件对结球甘蓝F2群体硫代葡萄糖苷总含量进行QTL定位和分析。最终检测到3个控制甘蓝硫代葡萄糖苷含量性状的QTLs(分别位于LG7上的 qGS-7-1 和位于LG8上的 qGS-8-1qGS-8-2),其贡献率分别为13.6%,7.5%,5.6%。并得到同时控制硫代葡萄糖苷含量QTLs共分离的分子标记me27-em25-2和E38M55-7,均可为辅助选育富含硫代葡萄糖苷甘蓝品种提供理论依据。

本文引用格式

田多成 , 何洪巨 , 严慧玲 , 颉建明 , 康俊根 . 甘蓝硫代葡萄糖苷总量性状的QTL定位及分析[J]. 华北农学报, 2014 , 29(3) : 6 -10 . DOI: 10.7668/hbnxb.2014.03.002

Abstract

Glucosinolates, anticancer active substances, are widely present in Brassica crops.It will be of important significance to carry out the breeding of high content of Glucosinolates new varieties of cabbage.This study analyzed total glucosinolate content of F2 mapping population parents and that of 83 individual plants by HPLC methods.Based on the cabbage high-density genetic linkage map which had been constructed previously, QTL mapping and analysis related to Glucosinolates total content in the individuals of F2 were performed using MapQTL 4.0.Three QTLs(qGS-7-1, qGS-8-1, qGS-8-2) located on LG7 and LG8 controlling the traits of Glucosinolates total content in cabbage were detected.The contribution rate were 13.6%, 7.5% and 5.6%, respectively.Meanwhile, two molecular markers(me27-em25-2, E38M55-7) coseparating with the QTLs could be useful for marker assisted selection of cabbage breeding rich in Glucosinolates.

参考文献

[1] 农业部.2006年全国各地蔬菜播种面积和产量[J].中国蔬菜,2008(1):65-66.
[2] 李 雷,邹 翔,季宇彬.十字花科植物中异硫氰酸盐的性质及活性研究[J].哈尔滨商业大学学报:自然科学版,2007,23(4):385-389,399.
[3] Fahey J W,Zalcmann A T,Talalay P.The chemical diversity and distribution of glucosinolates and isothiocyanates among plants[J].Phytochemistry,2001,56(1):5-51.
[4] Kushad M M,Brown A F,Kurilich A C,et al.Variation of glucosinolates in vegetable crops of Brassica oleracea[J].J Agric Food Chem,1999,47(4):1541-1548.
[5] Rosa E,Heaney K R,Fenwick R,et al.Glucosinolates in cropplants[J].Hort Rev,1997,19:99-215.
[6] 孙秀波,幕美财,李玫瑰,等.十字花科蔬菜硫代葡萄糖苷含量比较[J].安徽农学通报,2007,13(19):64-65.
[7] Cohen J H,Kristal A R,Stanford J L.Fruit and vegetable in takes and prostate cancer risk[J].J Nat l Cancer Inst,2000,92:61-68.
[8] Keck A S,Finley J W.Cruciferous vegetables:cancer protective mechanisms of glucosinolate hydrolys is products and selenium[J].Integr Cancer Ther,2004,3:5-12.
[9] Stoewsand G S,Anderson J L,Munson L.Protective effect of dietary brussels sprouts against mammary carcinogenes is in Sprague-Daw leyrats[J].Cancer Lett,1988,39:199-207.
[10] Wang L G,Liu D L,Ahmed T,et al.Targeting cell cycle machinery as a molecular mechanism of sulforaphane in prostate cancer prevention[J].Internat J On co l,2004,24:187-192.
[11] Wattenberg L W.Inhibition of carcinogenic effects of polycyclic hydrocarbons by benzyl isothiocyanate and related compounds[J].J Nat l Cancer Inst,1977,58(2):395-398.
[12] 何洪巨,陈 杭,Schnitle W H.芸薹属蔬菜中硫代葡萄糖苷鉴定与含量分析[J].中国农业科学,2002,35(2):192-197.
[13] 李 鲜,陈昆松,张明方,等.十字花科植物中硫代葡萄糖苷的研究进展[J].园艺学报,2006,33(3):675-679.
[14] Gao Muqiang,Li Genyi,Yang Bo,et al.Carlos guiros high-density Brassica oleracea linkage map:identification of useful new linkages[J].Theor Appl Genet,2007 115(2):277-287.
[15] 李占省,刘玉梅,方智远,等.青花菜DH群体花球中莱菔硫烷含量的遗传效应分析[J].园艺学报,2012,39(1):101-108.
[16] 陈健美,管荣展.播娘蒿种子中几种硫苷的提取及鉴定[J].西北植物学报,2006,26(6):1231-1235.
[17] 王丽红,吴莉莉,武冬梅,等.HPLC内标法测定猫耳朵菜的硫苷含量[J].北方园艺,2012(12):128-130.
[18] 朱洪运,颉建明,郁继华,等.结球甘蓝AFLP、SSR、SRAP标记高密度遗传图谱构建[J].华北农学报,2013,28(3):82-87.
[19] McCouch S R,X Chen,O Panaud,et al.Microsatellite marker development,mapping and applications in rice genetics and breeding[J].Plant Mol Biol,1997,35:89-99.
[20] 程 坤,仪登霞,杨丽梅,等.甘蓝硫代葡萄糖苷含量与其相关基因表达关系[J].园艺学报,2011,38(增刊):2547.
[21] 陈丽静,王 利,王玉坤,等.利用重组自交系群体构建番茄AFLP遗传连锁图谱[J].园艺学报,2012,39(12):2377-2384.
[22] 郝晓芬,王志民,王根全,等.SSR方法标记谷子光敏雄性不育基因[J].华北农学报,2011,26(5):112-116.
[23] 于栓仓,王永健,郑晓鹰.大白菜分子遗传图谱构建[J].中国农业科学,2003,36(2):190-195.
文章导航

/