为筛选木质素高效降解菌，对筛选的方法体系进行了研究。结果表明，利用三种定性筛选平板结合定量的木质素磺酸钙降解的方法，能明显提高筛选效率，从16个菌株中筛选到3株对木质素有较强降解能力的菌株Tp1、Tf1与Ls21。为进一步验证所筛菌株的降解效果，选取其中综合性能较好的Tf1菌株(凤尾菇，Pleurotus sajor-caju)，对小麦秸秆与玉米秸秆进行固态发酵试验，并以黄孢原毛平革菌(Phanerochaete chrysosporium)BKMF-1767作参照，结果表明，Tf1对小麦秸秆与玉米秸秆木质素的降解率分别为38．4%与47．7%，均高于参照菌株(分别为32．2%和34．6%)。
关键词：
秸秆; 木质素; 筛选; 降解; 白腐菌
To use three kinds of screening plate,and to combine with quantitative degradation test of calcium lignosulphonate,three lignin-degradation strains,TP1,Tf1 and Ls21 were obtained.In order to confirm the degradation effect of the strains,the strain Tf1 (Pleurotus sajor-caju),was selected to do solid substrate fermentation test about the wheat straw and corn straw.And Phanerochaete chrysosporium (BKMF-1767) was made as a reference.The result showed that the degradation rate of Tf1 to the wheat straw and corn was 38.4% and 47.7% respectively,and it was higher than the reference strain (32.2% and 34.6%).This research improves the screening method system of lignin-degrading fungus.There is an important significance to expand the resource of lignin-degrading fungus and exploit the straw resource.
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