摘要： 为研究在不同逆境,如低钾、低钙、NaCl及ABA胁迫下,对拟南芥高亲和性钾转运体基因 AtHAK5 表达的影响,在对含有 promoter AtHAK5::GUS 融合基因的拟南芥转基因植株进行组织化学染色基础上进行Real time RT-PCR检测。结果表明,这些逆境条件可以引起拟南芥 AtHAK5 基因表达量的上调。同时在对拟南芥Col-0和 AtHAK5 缺失突变体 athak5 表型对比分析,发现 AtHAK5 参与植物根对盐胁迫及ABA的反应。

关键词: AtHAK5, 拟南芥, 非生物胁迫

Abstract: In present study,the expression of AtHAK5 gene were found to be induced by some abiotic stress,such as low potassium,low calcium,NaCl and ABA by GUS activity based histochemical staining of the Arabidopsis thaliana transgenic plants containing a promoter AtHAK5::GUS fusion gene.These stress could up-regulate the expression of AtHAK5 gene of Arabidopsis thaliana confirmed by Real time RT-PCR.The differences of growth phenotype between wild-type Arabidopsis thaliana (Col-0)and the mutant(athak5)in the various abiotic stress treatments proved that AtHAK5 participated in the response to salt and ABA in the plant root.

Key words: AtHAK5, Arabidopsis thaliana, Abiotic stress

中图分类号: 

• S143.3