华北农学报 ›› 2011, Vol. 26 ›› Issue (4): 27-31. doi: 10.7668/hbnxb.2011.04.004

所属专题: 水稻

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水稻类受体激酶OsBRR1对稻瘟病抗性分析

李亚栋1,2, 何近刚1,3   

  1. 1. 河北农业大学, 河北保定 071001
    2. 中国农业科学院作物科学研究所, 北京 100081
    3. 河北省农林科学院遗传生理研究所, 河北石家庄 050051
  • 收稿日期:2011-03-20 出版日期:2011-08-28
  • 作者简介:李亚栋(1983-),男,河北邯郸人,在读博士,主要从事作物抗病育种研究。

The Diseases Resistance Analysis of OsBRR1,A Putative Rice Receptor Like Kinase

LI Ya-dong1,2, HE Jin-gang1,3   

  1. 1. Hebei Agricultural University, Baoding 071001, China;
    2. Institute of Crops, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    3. Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China
  • Received:2011-03-20 Published:2011-08-28

摘要: 基因OsBRR1是LRR-RLKs基因家族中的一员,LRR-RLKs的成员大多参与调控植物的生长发育以及防卫反应。本研究分别对OsBRR1的转基因干涉株系和超表达株系进行了稻瘟病抗病鉴定。结果显示,转基因干涉株系比转空载体和野生型对照株系对具有中等毒性的稻瘟病菌株研54-04显示更强的感病性;而超表达株系对三种不同的强致病力菌株97-27-2、99-31-1与中10-8-14具有显著的抗性。结果表明,OsBRR1的表达与水稻对稻瘟病的抗性密切相关,参与调控水稻对稻瘟病的抗性。

关键词: 类受体激酶, 稻瘟病抗性, RNA干涉, 超表达

Abstract: OsBRR1 is a novel member of leucine-rich repeat receptor-like kinases family,most of which regulate a wide variety of developmental and defense-related processes.In this study,evaluation of disease resistance was performed separately to the lines of OsBRR1-suppression plants as well as overexpressing plants.Wild-type plants showed intermediate resistance to weakly virulent isolate of Magnaporthe oryzae,Ken 54-04,while OsBRR1-suppression plants were susceptible to Ken 54-04.Furthermore,OsBRR1-overexpressing plants exhibited enhanced resistance to some virulent isolates(97-27-2,99-31-1 and Zhong 10-8-14).These results indicate that OsBRR1 is involved in rice resistance responses to blast fungus and mediates resistance to rice blast.

Key words: Receptor-like kinase, Rice blast resistance, RNA interference, Overexpression

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引用本文

李亚栋, 何近刚. 水稻类受体激酶OsBRR1对稻瘟病抗性分析[J]. 华北农学报, 2011, 26(4): 27-31. doi: 10.7668/hbnxb.2011.04.004.

LI Ya-dong, HE Jin-gang. The Diseases Resistance Analysis of OsBRR1,A Putative Rice Receptor Like Kinase[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 27-31. doi: 10.7668/hbnxb.2011.04.004.

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