摘要： 用纯化的猪伪狂犬病病毒闽A株(PRV-FA)免疫Balb/c鼠,取脾细胞和骨髓瘤细胞进行细胞融合,经间接ELISA筛选,获得2株能稳定分泌伪狂犬病毒单克隆抗体的杂交瘤细胞株,分别命名为4G9E9和4H9C9。这2株杂交瘤细胞株细胞培养上清和小鼠腹水效价(ELISA)分别为1∶6400、1∶12800及1∶12800、1∶25600。特异性鉴定结果表明,各株单抗均不与猪瘟病毒、乙脑病毒、猪呼吸繁殖障碍综合症病毒、猪细小病毒等发生交叉反应,这2株抗PRV杂交瘤细胞株的获得为进一步建立准确快速的抗原检测方法奠定了基础。

关键词: 伪狂犬病病毒, 单克隆抗体, 制备

Abstract: The purified psudorabies virus strain FA(PRV -A)was inoeulated to Balb/c mice.Two m -onoclonal hybridona cell named 4G9E9 and 4H9G9 lines which were able to stably exerete monoclonal antibodies against PRV were produced by means of hybridoma technique and indirect ELISA detection.The indirect ELISA titer of culture supernatant and ascites were 1∶6 400,1∶12 800 and 1∶12 800,1∶25 600.All these monoclonal antibodies didn′t react with HCV,JEV,PRRSV and PPV.These results could be used to develop diagnostic methods for detection of PRV antigen as well as to investigate protein function of PRV.

Key words: Psudorabbiesvirus, Monoclonalantibodies, Preparation

中图分类号: 

• Q785