摘要： 针对磨盘柿杂种胚挽救培养后的幼苗侧根少、直接成苗率低这一问题，采用对比法设计研究了在后续培养中几种措施的生根壮苗效应。结果表明：在原萌发培养基中添加1%墨汁可使胚挽救培养的直接成苗率提高到87%以上；对不能直接驯化移栽的胚挽救苗，短截主根和无糖培养措施能促进其侧根发育，并提高了幼苗的自养能力；3种措施的综合运用最终可使96%以上的杂种胚得以挽救成苗，并成功移栽于大田。

关键词: 磨盘柿, 胚挽救, 生根壮苗培养

Abstract: Rooting and hardening-off culture were specially performed to solve some problems emerged in previous embryo rescue of Chinese persimmon cv. Mopanshi. The contrast designs showed that adding 1% Chinese ink in the basic medium for germination could raise the percentage of plantlet formation by 87%. In hardening-off culture stage, cutting the main root and sucrose-free culture could improve the development of lateral roots and the nutrient acclimation of hybrid plantlets. By combining use of above three measures, more than 96% embryos were successfully rescued and transplanted to field.

Key words: Mopanshi persimmon, Embryo rescue, Rooting and hardening-off culture

中图分类号: 

• S665.2