氯甲基蛋氨酸缓解脂多糖诱导的IPEC-J2细胞凋亡及其机制

doi:10.7668/hbnxb.20195572

摘要/Abstract

摘要：

旨在探讨氯甲基蛋氨酸(MMC)对脂多糖(LPS)诱导的仔猪空肠上皮细胞IPEC-J2凋亡及相关蛋白表达的影响,并解析其潜在的作用机制。通过设立空白组、模型组(LPS处理)和试验组(MMC+LPS处理),分析MMC对LPS诱导的IPEC-J2细胞的活力、凋亡率、迁移数及凋亡相关蛋白的影响。结果显示,试验组相较于模型组,细胞活力显著提升了18%,迁移数极显著提升了约2倍,凋亡率极显著降低了40%。MMC抑制了LPS诱导的JNK通路激活,表现为p-JNK蛋白表达水平极显著下降25.5%。进一步分析表明,MMC显著降低了促凋亡蛋白Cleaved-caspase-3和BAX的表达,分别下降了31.5%,26.3%,同时显著提高了抗凋亡蛋白BCL-2的表达水平。综上,氯甲基蛋氨酸通过调控凋亡相关蛋白表达、抑制JNK通路激活,有效缓解LPS诱导的IPEC-J2细胞凋亡,为氯甲基蛋氨酸在猪肠道健康保护中的应用提供了科学依据。

关键词: 氯甲基蛋氨酸, 脂多糖, IPEC-J2细胞, 细胞活力, 细胞凋亡

Abstract:

This study aimed to investigate the effect of DL-methionine methylsulfonium chloride(MMC)on apoptosis and the expression of related protein genes in lipopolysaccharide(LPS)-induced porcine jejunal epithelial cells(IPEC-J2),and to elucidate its potential mechanism of action.By setting up a blank group,a model group(LPS-treated)and an experimental group(MMC+LPS-treated),the study compared the effects of MMC on the viability,apoptosis rate,migration number and apoptosis-related proteins of LPS-induced IPEC-J2 cells.It was found that the experimental group significantly increased cell activity by 18% and migration number by about 2 times,and significantly decreased apoptosis rate by 40% compared with the model group.Additionally,MMC also inhibited LPS-induced activation of the JNK pathway,as evidenced by a highly significant decrease of 25.5% in the p-JNK protein expression level in the LPS model group.Further analysis showed that MMC significantly reduced the expression of pro-apoptotic proteins Cleaved-caspase-3 and BAX,which decreased by 31.5% and 26.3% respectively in the LPS model group,while it markedly increased the expression level of the anti-apoptotic protein BCL-2.In conclusion,MMC effectively alleviates LPS-induced apoptosis in IPEC-J2 cells by regulating the expression of apoptosis-related proteins and inhibiting JNK pathway activation,which provides a scientific basis for the application of MMC in protecting intestinal health in pigs.

Key words: DL-methionine methylsulfonium chloride, Lipopolysaccharide, IPEC-J2 cells, Cell viability, Cell apoptosis

中图分类号:

S816.71无机(矿物质)饲料

姜东凤, 乔英英, 杨建平, 聂芙蓉, 李蕊, 王可, 雷蕾, 张卫宪. 氯甲基蛋氨酸缓解脂多糖诱导的IPEC-J2细胞凋亡及其机制[J]. 华北农学报, 2025, 40(4): 232-238. doi: 10.7668/hbnxb.20195572.

JIANG Dongfeng, QIAO Yingying, YANG Jianping, NIE Furong, LI Rui, WANG Ke, LEI Lei, ZHANG Weixian. The Mechanism of DL-Methionine Methylsulfonium Chloride Alleviating Lipopolysaccharide-Induced Apoptosis in IPEC-J2 Cells[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(4): 232-238. doi: 10.7668/hbnxb.20195572.

http://www.hbnxb.net/CN/Y2025/V40/I4/232

图/表 7

图1 MMC对LPS诱导的IPEC-J2细胞活力的影响不同大写字母表示在0.01水平上差异显著,不同小写字母表示在0.05水平上差异显著。图3,5,6,7同。

Fig.1 The effect of MMC on the viability of LPS-induced IPEC-J2 cells Different uppercase letters indicate significant differences at the 0.01 level,different lowercase letters indicate significant differences at the 0.05 level.The same as Fig.3,5,6,7.

图2 MMC对LPS诱导的IPEC-J2细胞凋亡影响流式图 A.空白组;B.LPS模型组;C.MMC+LPS试验组。

Fig.2 Flow cytometry analysis of the effect of MMC on apoptosis in LPS-induced IPEC-J2 cells A.Blank group;B.LPS model group;C.MMC+LPS treatment group.

图3 MMC对LPS诱导的IPEC-J2细胞凋亡的影响

Fig.3 The effect of MMC on apoptosis in LPS-induced IPEC-J2 cells

图4 MMC处理下LPS诱导的IPEC-J2细胞迁移图 A.空白组;B.LPS模型组;C.MMC+LPS试验组。

Fig.4 Migration analysis of IPEC-J2 cells induced by LPS and treated with MMC A.Blank group;B.LPS model group;C.MMC+LPS treatment group.

图5 MMC对LPS诱导的IPEC-J2细胞迁移的影响

Fig.5 The effect of MMC on the migration of LPS-induced IPEC-J2 cells

图6 不同处理对IPEC-J2细胞JNK和p-JNK表达量的影响 1.空白组;2.LPS模型组;3.MMC+LPS试验组。图7同。

Fig.6 The effect of different treatments on JNK and p-JNK expression in IPEC-J2 cells 1.Blank group;2.LPS model group;3.MMC+LPS treatment group.The same as Fig.7.

图7 不同处理对凋亡相关蛋白表达量的影响

Fig.7 The effect of different treatments on the expression of apoptosis-related proteins

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