大枣不同组织氮稳定同位素变化规律研究

doi:10.7668/hbnxb.2015.S1.078

摘要/Abstract

摘要： 为了打击农产品造假,提高农产品质量安全,急需建立特色农产品的溯源数据库.数据库的建立,首先要建立溯源指标体系.以河北省太行山区大枣主产区赞皇县、行唐县、阜平县的赞皇大枣和婆枣为研究对象,测定其皮、肉、核3个组织中的δ¹⁵N值,以期明确大枣各组织中N同位素的变化规律,以及品种、地域、采摘时期等因素对大枣δ¹⁵N值的作用,筛选出大枣与产地溯源相关的取样部位.结果发现:在品种、地域、采摘时期、年份等各因素综合影响下,δ¹⁵N值在赞皇大枣和婆枣品种间存在差异,赞皇大枣高于婆枣;N同位素在赞皇大枣和婆枣不同组织的变化规律并不相同:赞皇大枣:枣核>枣肉(P>0.05)>枣皮(P>0.05),婆枣:枣肉 >枣皮(P>0.05) >枣核(P<0.05).枣肉中氮同位素品种间差异不显著,因此,在进行大枣的产地溯源时,建议选取枣肉作为溯源取样部位.品种、地域、采摘时期三因素对大枣枣肉的δ¹⁵N值均具有协同作用.

关键词: 大枣, 溯源, 稳定同位素, &delta, ¹⁵N

Abstract: For the reason that:some agricultural products which have higher economic value were often simulated by the counterfeiters.It was in urgent need of building theirs source database to insure the safety of agricultural products in order to clear the change laws of stable nitrogen isotope in tissue of Chinese-date,and the effect of such factors as breed,region,and sampling time on δ¹⁵N values of in tissue of Chinese-date,as well as to screen the sampling location of Chinese-date in origin traceability,it was measured in this paper δ¹⁵N values in tissue of fructus corni,jujube meat,jujube pit as the research object to Zanhuang jujube and jujube in Tai-hang Mountain main producing areas such as Zanhuang Country,Xingtang Country,Fuping Country.The results showed that:Under the comprehensive influence of such factors as breed,region,sampling time and year,the difference of δ¹⁵N value sexsited between breeds of Zanhuang jujube and jujube,Zanhuang jujube was higher than that of jujube;And the change law of the two breeds was different:For Zanhuang jujube,jujube pit >jujube meat(P>0.05) >frctus corni(P>0.05),For jujube:jujube meat>frctus corni(P>0.05)>jujube pit(P<0.05).For the reason that the value of δ¹⁵N had no significant difference in jujube meat between two breeds,it was suggested that we should take jujube meat as sampling location in origin traceability of Chinese-date.The effects of breed,region,and sampling time on δ¹⁵N of jujub meat was co-operational.

Key words: Chinese-date, Traceability, Stable isotope, δ¹⁵N

中图分类号:

S665.01
P597

王红云, 高占锋, 付才, 黄志国, 常志强, 刘悦. 大枣不同组织氮稳定同位素变化规律研究[J]. 华北农学报, 2015, 30(S1): 429-434. doi: 10.7668/hbnxb.2015.S1.078.

WANG Hong-yun, GAO Zhan-feng, FU Cai, HUANG Zhi-guo, CHANG Zhi-qiang, LIU Yue. Study on the Change Law of Stable Nitrogen Isotope in Tissue of Chinese-date[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 429-434. doi: 10.7668/hbnxb.2015.S1.078.

http://www.hbnxb.net/CN/Y2015/V30/IS1/429

参考文献

[1] 李沈轶,胡柳花,隋丽敏,等.利用内标碳同位素比率法研究蜂蜜中植物糖浆[J].食品工业科技,2010,31(7):365-367,370.
[2] 袁玉伟,张永志,付海燕,等.茶叶中同位素与多元素特征及其原产地PCA-LDA判别研究[J].核农学报,2013,27(1):47-55.
[3] 袁玉伟,胡桂仙,邵圣枝,等.茶叶产地溯源与鉴别检测技术研究进展[J].核农学报,2013,27(4):452-457.
[4] 朱绍华,张帆,王美玲,等.稳定同位素比质谱法鉴别茶油中掺杂玉米油研究[J].中国食物与营养,2013,19(3):8-10.
[5] 林植芳,孙谷畴,王伟,等.药用植物叶片的碳同位素比和矿质元素含量[J].植物学报,1989,31(5):367-375.
[6] 郭波莉,魏益民,Kelly D S,等.稳定性氢同位素分析在牛肉产地溯源中的应用[J].分析化学,2009(9):1333-1336.
[7] 郭波莉,魏益民,潘家荣.同位素指纹分析技术在食品产地溯源中的应用进展[J].农业工程学报,2007,23(3):284-289.
[8] 郭波莉,魏益民,潘家荣,等.碳、氮同位素在牛肉产地溯源中的应用研究[J].中国农业科学,2007,40(2):365-372.
[9] 郭波莉,魏益民,潘家荣,等.牛不同组织中稳定性碳同位素组成及变化规律研究[J].中国农业科学,2006,39(9):1885-1890.
[10] 王慧文,杨曙明,程永友.鸡肉中稳定同位素组成与饲料和饮水关系的研究[J].分析科学学报,2008,24(1):47-50.
[11] 孙淑敏,郭波莉,魏益民,等.羊组织中碳、氮同位素组成及地域来源分析[J].中国农业科学,2010,43(8):1670-1676.
[12] 孙丰梅,于洪侠,石光雨,等.牛组织中稳定性同位素碳、氮随饲料变化的研究[J].分析测试学报,2009,28(3):310-314.
[13] 孙丰梅,于洪侠,吴伟,等.稳定性同位素碳、氮在牛不同组织中的变化规律[J].核农学报,2009,26(3):462-466.
[14] 孙丰梅,石光雨,王慧文,等.牛不同组织中稳定性同位素氢、氧、硫组成探讨[J].核农学报,2012,26(8):1148-1153.
[15] 刘晓玲,郭波莉,魏益民,等.不同地域牛尾毛中稳定同位素指纹差异分析[J].核农学报,2012,23(2):330-334,329.
[16] 刘泽鑫,郭波莉,潘家荣,等.陕西关中地区肉牛产地同位素溯源技术初探[J].核农学报,2008,22(6):834-838.
[17] 董惟妙.黄土高原现代粟黍样品碳氮稳定同位素组成及炭化对同位素分馏的影响[D].兰州:兰州大学,2012.
[18] 董星彩,王颜红,李国琛,等.五味子稳定碳同位素分布特征及其与环境因子的关系[J].生态学杂志,2010,29(12):2353-2357.
[19] 钟敏.用碳氮稳定同位素对大米产地溯源的研究[D].大连:大连海事大学,2013.
[20] 黄岛平,陈秋虹,林葵,等.稳定碳氢同位素在柑桔产地溯源中应用初探[J].科技与企业,2013(17):256-257.
[21] Bateman A S,Kelly S D,Jickells T D.Nitrogen isotope relationships between crops and fertilizer:implications for using Nitrogen isotope analysis as an indicator of agricultural regime[J].Journal of Agricultural and Food Chemistry,2005,53(14):5760-5765.
[22] 胡新艳,王贵禧,梁丽松,等.不同肥料对冬枣果实品质发育的影响[J].林业科学研究,2007(6):750-754.
[23] 郑丽锦,王东晨,曹彦卫,等.有机肥对赞皇大枣生长和产量的影响[J].河北果树,2010(2):6-7.
[24] 王会.有机无机肥配施对蔬菜δ¹⁵N和土壤生物学性质的影响[D].泰安:山东农业大学,2012
[25] 诸葛玉平,苏志慧,张彤,等.北京郊区有机蔬菜土壤养分平衡及δ¹⁵N特征分析[J].农业环境科学学报,2011(11):2313-2318.
[26] 张彦昌,赵德英.果树氮素贮藏营养研究进展[J].山西农业科学,2009,37(1):88-91.
[27] 魏天军,李百云.采收期和品种对枣果实品质的影响[J].中国农学通报,2009,25(9):184-187.
[28] 冯美.落叶果树氮素营养研究[J].宁夏农林科技,2005(1):33-35.
[29] 周咏春,程希雷,樊江文.植物氮同位素组成与其影响因子的关系研究进展[J].草地学报,2012,20(6):981-989.
[30] Bergersen F,Peiples M,Turner G.Isotopic discriminations during the accumulation of Nitrogen by soybeans[J].Futional Plant Biology,1988,15(3):407-420.
[31] Evans R,Bloom A,Sukrapanna S,et al.Nitrogen isotope composition of tomato(Lycopersicon esculentum Mill.cv.T-5)grown under ammonium or nitrate nutrition[J].Plant,Celll & Environment,1996,19(11):1317-1323.
[32] Codron J,Codron D,Lee-Thorp J A,et al.Taxonomic,anatomical,and spatio-temporal variations in the stable Carbon and Nitrogen isotopic compositions of plants from an African savanna[J].Journal of Archaeological Science,2005,32(12):1757-1772.
[33] Sah S,Brumme R.Altitudinal gradients of natural abundance of stable isotopes of Nitrogen and Carbon in the needles and soil of a pine forest in Nepal[J].Journal of Froest Science,2003,49(1):19-26.
[34] Couto-Vázquez A,González-Prieto S J.Effects of climate,tree age,dominance and growth on δ¹⁵N Young Pine woods[J].Trees,2010,24(3):507-514.
[35] Nadelhoffer K J,Downs M R,Fry B.Sinks for 15N-enriched additions to an oak forest and a red pine plantation[ J].Ecological Applications,1999,9(1):72-86.
[36] Evans R D.Physiological mechanisms influencing plant Nitrogen isotope composition[J].Trends in Plant Science,2001,6(3):121-126.
[37] Tjepkema J D,Schwintzer C R,Burris R H,et al.Natural abundance of ¹⁵N in actinorhizal plants and nodules[J].Plant and Soil,2000,219(1/2):285-289.
[38] Michelsen A,Schmidt I K,Jonasson S,et al.Leaf 15N abundance of subarctic plants provides field evidence that ericoid,ectomycorrhizal and non-and arbuscular mycorrhizal species access different sources of soil Nitrogen[J].Oecologia,1996,105(1):53-63.
[39] Dawson T E,Mambelli S,Plamboeck A H,et al.Stable Isotopers in plant ecology[J].Annual Review of Ecology and Systematics,2002,33:507-559.
[40] Hobbie E A,Macko S A,Williams M.Correlations between foliar δ¹⁵N and nittrogen concentrations May indicate plant-mycorrhizal inter-actions[J].Oecologia,2000,122(2):273-283.
[41] 胡芳名,谢碧霞,刘佳佳,等.氮素营养对枣树花和果实生长发育的影响研究[J].经济林研究,1995(4):16-19,79.

选择文件类型/文献管理软件名称

选择包含的内容