华北农学报

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茶薪菇不同生长期几种胞外酶活性的测定

路等学, 王龙, 高静梅, 王秉峰, 张铎, 赵玉卉   

  1. 甘肃省科学院生物研究所, 甘肃兰州 730000
  • 收稿日期:2009-03-05 出版日期:2009-12-31
  • 作者简介:路等学(1962 -), 男, 高级工程师, 主要从事食用菌遗传育种、栽培技术等方面的研究工作。
  • 基金资助:
    甘肃省自然基金项目(3ZS061-A25-082)

Detecting in Several Extracellular Enzymes Activities of Agrocybe chaxingu Huang

LUDeng-xue, WANGLong, GAOJing-mei, WANGBing-feng, ZHANGDuo, ZHAOYu-hui   

  1. Institute of Biology, Gansu Academy of Sciences, Lanzhou 730000, China
  • Received:2009-03-05 Published:2009-12-31

摘要: 在常规栽培条件下,对茶薪菇8个不同生长发育阶段6种与培养料主要组分分解相关的胞外酶的变化情况进行了测定,结果表明:在相同培养料中,来源不同的茶薪菇菌株6种胞外酶只是酶活性的大小相对不同,并没有显著性差异;同时,酶活变化规律基本趋于一致,酶活性均随第一潮子实体的成熟出现一个活性峰值,在营养生长期,酶活性处于整体上升趋势,生殖生长阶段则呈下降趋势,部分酶活性后期基本丧失。

关键词: 茶薪菇, 生长期, 胞外酶

Abstract: Under normal cultivation conditions,dynamic variation law of six kinds of extraccellular enzyme activitieswhich were relative to cultivation composition was investigated during the whole growth period of Agrocybe chaxinguHuang.The results revealed that five different sources strains were only relative discrepancy of enzyme activities in thesame medium compositions,not the variation law of enzyme activities, there was an active peak following the maturation offruitbodies in each flush for most of the enzymes,and that in general,the enzyme activities increased during myceliumgrowth stage,decreased during fruiting stage,and part of enzymes lost their activities in later period of cultivation.

Key words: AgrocybechaxinguHuang, Growthstage, Extracellularenzymes

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引用本文

LUDeng-xue, WANGLong, GAOJing-mei, WANGBing-feng, ZHANGDuo, ZHAOYu-hui. Detecting in Several Extracellular Enzymes Activities of Agrocybe chaxingu Huang[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.064.