摘要： 以秋水仙素为诱变剂,利用茎段浸泡和秋水仙素培养基2种方法对库拉索芦荟进行诱变,结果表明,茎段浸泡秋水仙素法诱导四倍体葡萄的效果优于秋水仙素培养基法,0.2%的秋水仙素处理4 d变异效果最好,诱变率达到最高值41.5%。变异株叶片肥厚,叶色深绿,叶片表皮气孔保卫细胞中叶绿体数增多。

关键词: 芦荟, 秋水仙素, 多倍体

Abstract: Tetraploids were induced from in vitro Aloe vera L by soaking shoot segments in colchicine solution and adding colchicine in the culture medium.The results showed that the adding colchicine in the culture medium increased the efficiency of induced tetraploids than one of soaking treatment.The optimal conditions for inducing tetraploids were adding colchicine in the culture medium for 3 to 4 d with colchicine at a concentration of 2 000 mg/L.The rate of induction was 41.5%.Compared with the diploids,the appearance of variations is distinct including plumper leaves and more chloroplasts in stomatal guard cells.

Key words: Aloe vera L, Colchicines, Multiploid

中图分类号: 

• S682.01