摘要： Preliminary study has been made to test wether three strains McAb（1B1,5D6, 6D8）are against the same antigen determinant.Througn ELISA additive and competition expeiments,itproved that these three strains are against different antigen determinant.The result of positiveserum antigen component analysis with 2 strains IBDV McAb showed that sample IBD positiveserum had obvious inhibition against combination of 5D6 McAb with corresponding antigen.Theresults of substitution of corresponding component in ELISA inhibition experiment and compari-son of non-IBD serum （SPF chicken serum,ND,MD,IA positive serum）proved that IBD anti-serum was the only one showing inhibition against 5D6 McAb.Comparison with AGP and electro-microscope observation showed that ELISA inhibition experiment was characterised by high-specificity,rapidity and sensitiveness.799 serum samples were tested with ELISA inhibition anddouble immunodiffusion（AGP）experiments.
关键词:
infectious bursal disease,
virus,
monoclone,
chicken
乔忠, 詹丽娥, 史振心, 马丛林, 刘(王子), 宁官宝. Application of monoclonal antibodies against chicken infectious bursal disease virus(IBDV)[J]. 华北农学报, 1993, 8(S2): 135-139. doi: 10.7668/hbnxb.1993.S2.024.
Qiao Zhong, Zhan Lie, Shi Zhenxin, Ma Conglin, Liu Zi, Ning Guanbao. [J]. ACTA AGRICULTURAE BOREALI-SINICA, 1993, 8(S2): 135-139. doi: 10.7668/hbnxb.1993.S2.024.