摘要： This paper reports a new transgenic tobacco line NC89 resistant to both tobaccomosaic virus(TMV)and cucumber mosaic virus(CMV).It deals with the details of field experi-ments of the whole process of segregation and selection of the progenies of transgenic plant popu-lation.By using the gene transfer technics,some transgenic plants resistant to mosaic disease froma flue-cured tobacco cultivar NC89 were obtained in green house in 1989.Thereafter,throughfield inoculation and selection from the 2nd and 3rd generations,a homozygous line resistant toboth viruses and with good qualities was bred out.The inheretant stability of the resistance of thenew line was confirmed through the 4th to 6th generations.In 1992,the transgenic resistant linewas planted about 7000 hectares in Henan province.The relative effect of controlling mosaic dis-eases of tobacco was 50—70%.The out-put value increased 30—50% per unit area.This is a newexample of growing a transgenic virus-resistant Plant in large scale in China.
关键词:
gene-transfer,
tobacco mosaic virus,
cucumber mosaic virus,
new transgenictant line of tobacco
周汝鸿, 张振臣, 吴青, 孙清, 方荣祥, 莽克强, 田颖川, 王桂玲, 侯文华, 李克治, 董继超, 杨静. The technics of breeding a new transgenic tobacco cultivar resistant to mosaic diseases and its extention[J]. 华北农学报, 1993, 8(S2): 58-63. doi: 10.7668/hbnxb.1993.S2.011.
Zhou Ruhong, Sun Qing, Fang Rongxiang, Tian Yingchuan, Hou Wenhua, Yang Jing, Zhang Zhenchen, Wu Qing, Mang Keqiang, Wang Guiling, Li Kezhi, Dong Jichao. [J]. ACTA AGRICULTURAE BOREALI-SINICA, 1993, 8(S2): 58-63. doi: 10.7668/hbnxb.1993.S2.011.