泌盐植物长叶红砂质膜Na<sup>+</sup>/H<sup>+</sup>逆向转运蛋白基因(RtSOS1)全长cDNA的克隆及序列分析

doi:10.3969/j.issn.1000-7091.2013.03.001

华北农学报 ›› 2013, Vol. 28 ›› Issue (3): 1-6. doi: 10.3969/j.issn.1000-7091.2013.03.001

所属专题： 盐碱胁迫；生物技术；

• 论文 • 下一篇

泌盐植物长叶红砂质膜Na⁺/H⁺逆向转运蛋白基因(RtSOS1)全长cDNA的克隆及序列分析

党振华, 郑琳琳, 冯智, 王迎春

内蒙古大学生命科学学院内蒙古自治区牧草与特色作物生物技术重点实验室, 内蒙古呼和浩特 010021

收稿日期:2013-04-22 出版日期:2013-06-28
通讯作者: 工迎春(1966-)，女，内蒙古呼和浩特人，博士，教授，主要从事植物生理生态及分子生物学研究
作者简介:党振华(1982-)，男，内蒙古包头人，在读博士，主要从事植物抗逆分子生物学研究
基金资助:
国家自然科学基金主任基金项目(31140020);内蒙古自然科学基金重大项目(2012ZD05)

Cloning and Sequence Analysis of the Plasma Membrane Na⁺/H⁺Antiporter cDNA in Recretohalophyte Reaumuria trigyna Maxim.

Dang Zhenhua, Zheng Linlin, Feng Zhi, Wang Yingchun

Inner Mongolia University College of Life Sciences,Key Laboratory of Herbage & Endemic Crop Biotechnology in Inner Mongolia, Huhhot 010021,China

Received:2013-04-22 Published:2013-06-28

摘要/Abstract

摘要： 长叶红砂为内蒙古东阿拉善-西鄂尔多斯地区特有珍稀泌盐,强旱生小灌木,对盐渍荒漠环境具有极强适应性。利用RT-PCR和RACE技术从该植物中分离出质膜Na⁺/H⁺逆向转运蛋白基因(RtSOS1),该cDNA全长为3 829bp,开放阅读框为3 438 bp,编码一个含1 145个氨基酸的蛋白质,推测分子量为126.76 kDa。氨基酸序列的生物信息学分析推测,该蛋白N端含有11个跨膜结构域,C端为一个长约700个氨基酸的亲水性尾,具有磷酸化和自我抑制结构域。同源性比对和系统发育分析证实,RtSOS1与其他植物的质膜Na⁺/H⁺逆向转运蛋白亲缘关系较近。

关键词: 长叶红砂, 质膜Na⁺/H⁺逆向转运蛋白基因, 全长cDNA克隆

Abstract: Reaumuria trigyua Maxim. is an endangered small shrub with the features of a recretohalophyte. This species is endemic to the Eastern Alxa Western Ordos area and developed distinctive strategies to adapt to the semidesert and salty soil environment. A full}ength cDNA of the plasma Na⁺/H⁺antiporter (RtSOSl)was isolated from this species by using RT-CR and RACE technologies. The 3 829 by sequence comprised a 3 438 by open reading frame，encoding an 1 145 amino acids protein with the molecular weight of 126.76 kDa. Bioinformatics analyze reveals that RtSOSl composed of 11 transmembrane domains within its N terminal portion，and a hydrophilic cytoplasmic tail with the length approximately 700 amino acids in its Germinal portion. In the Germinal region，the phosphorylation domain and the auto-nhibited domain are found. The Homology comparison and phylogenetic analysis showed that RtSOSl is related to plasma membrane Na⁺/H⁺antiporter in other plant species.

Key words: Reaumuria trigyua Maxim, SOSl, cDNA cloning

中图分类号:

党振华, 郑琳琳, 冯智, 王迎春. 泌盐植物长叶红砂质膜Na⁺/H⁺逆向转运蛋白基因(RtSOS1)全长cDNA的克隆及序列分析[J]. 华北农学报, 2013, 28(3): 1-6. doi: 10.3969/j.issn.1000-7091.2013.03.001.

Dang Zhenhua, Zheng Linlin, Feng Zhi, Wang Yingchun. Cloning and Sequence Analysis of the Plasma Membrane Na⁺/H⁺Antiporter cDNA in Recretohalophyte Reaumuria trigyna Maxim.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 1-6. doi: 10.3969/j.issn.1000-7091.2013.03.001.

http://www.hbnxb.net/CN/Y2013/V28/I3/1

参考文献

[1] Zhu .T K. Plant salt tolerance [J]. Trends in Plant Science,2001,6(2):66-71
[2] Shi H. The Arabidopsis thaliana salt tolerance gene SOSl encodes a putative Na⁺/H⁺antiporter [J]. Proceedings of the National Academy of Soiences , 2000 , 97(12):6896-6901.
[3] 马清，包爱科，伍国强，等质膜Na⁺/H⁺逆向转运蛋白与植物耐盐性[J].植物学报，2011, 46 (2):206-215.
[4] Oh D H , Ali Z , Hyeong C P ,et al. Consequences of SOSl defioiency:intracellular physiology and transcription[J] Plant Signal Behavior,2010,5(6):766-768.
[5] Oh D H ,Lee S Y, Bressan R A, et al. Intracellular consequenc;es of SOSl defioienc;y during salt stress [J] . Journal of Experimental Botany,2010,61 (4):1205-1213
[6] Wu Y,Ding N, Zhao X,et al. Molecular charac;terization of PeSOSl:the putative Na⁺/H⁺antiporter of Populu.s euphratica [J] . Plant Molecular Biology,2007 ,65(1):1-11
.
[7] Xu H , Jiang X,Zhan K , et al. Functional charac;terization of a wheat plasma membrane Na+/H+antiporter in yeast[J]. Archives of Biochemistry and Biophysic，2008，473(1):8-15.

[8] Mart nez 4tienza J, Jiang X ,Garoiadeblas B ,et al. Conservation of the salt overly sensitive pathway in rice[J]Plant Physiology,2007，143 (2):1001-1012.
[9] Ratner A , Jac;oby B. Effect of K+，its counter anion, and pH on sodium efflux from barley root tips [J] . Journal of Experimental Botany,1976 , 27 (5):843-852.
[10] Olias R, Eljakaoui Z, Li J, et al. The plasma membrane Na⁺/H⁺antiporter SOS1 is essential for salt tolerance in tomato and affects the partitioning of Na+between plant organs[J].Plant Cell&Environment 2009，32(7):904-916.
[11] Zhou G A ,Jiang Y ,Yang Q ,et al. Isolation and characterization of a new Na⁺/H⁺antiporter gene O.5WCAI from rice(Oryza .saliva L) [J] Mitochondrial DNA 2006,17(1):24-30.
[12] Wu C, Gao X , Kong X,et al. Molecular cloning and functional analysis of a Na+/H+antiporter gene ThNH 1from a halophytic plant Thellmagiella halophila [J] . Plant Molecular Biology Reporter,2009,27(1):1-12.
[13] Cosentino C,Fisc;her S E, Bertl A,et al. Na⁺/H⁺antiporters are differentially regulated in response to NaCI stress in leaves and roots of Me.sembrya}athemum cry.stalliaum [J] . New Phytologist, 2010 186(3):669-680.
[14] Yadav N S, Shukla P S ,Jha A,et al. The SbSOSl gene from the extreme halophyte Salicoraia brachiata enhances Na⁺loading in xylem and confers salt tolerance in transgenic tobacco [J] . BMC Plant Biology 2012 12:1RR.
[15] 王鹤，张高华，王旭达，等嶂茅耐盐基因sosl的克隆及序列分析[J].中国农业大学学报，2012, 17(3):28-33.
[16] 赵祥强玉米Na⁺/H⁺逆向转运蛋白基因ZmSOSI的克隆与鉴定田安徽农业科学，2009 ,37 (35):17843-17848.
[17] 马苏勇，祝建波，王爱英，等大叶补血草质膜Na⁺/H⁺逆向转运蛋白基因SOSI的克隆及对番茄的遗传转化日]生物技术通报，2010,36(9):1353-1358.
[18] 王澎，任晴雯，樊国盛黄瓜质膜型Na⁺/H⁺逆向转运蛋白基因(SOSI)的克隆与序列分析[J].上海交通大学学报:农业科学版，2012,30 (03):6-10.
[19] 玉祥星星草质膜型Na⁺/H⁺逆向转运蛋白基因的克隆和特性分析[J] 植物生理学通讯，2008,44(01):59-64
[20] Flowers T J, Colmer T D. Salinity tolerance in halophytes [J] . New Phytologist,2008，179 (4):945-963 Turkan I , Demiral T. Recent developments in understanding salinity tolerance [J] . Environmental and Experimental Botany,2009 ,67(1):2-9.
[22] 薛焱，王迎春，王同智濒危植物长叶红砂适应盐胁迫的生理生化机制研究田西北植物学报，2012,32(1):136-142.
[23] Dang Z H, Zheng L LWang J ,et ul. Transc riptomic profiling of the salty tress response in the wild receretohalophyte Reuumuriu trigyuu [J] . BMC Genomic 2013，14:29.
[24] 薛焱，王迎春.盐生植物长叶红砂泌盐特性的研究田中国沙漠,2008,28 (3):437-442.
[25] Shi H , Lee BWu S J，et ul. Overexpression of a plasma membrane Na⁺/H⁺antiporter gene improves salt tolerance in Arubidop.si,s thuliuuu [J] . Nature Biotechnology 2002,21(1):81-85.
[26] .
Quintero F J ,Martinez Atienza J,Villalta I ,et al. Activation of the plasma membrane Na+/H+antiporter Salt 0verly Sensitive 1 ( SOSl ) by phosphorylation of an autoinhibitory C terminal domain [J]. Proceedings of the National Academy of Soiences ,2011,108 (6):2611-2616.

选择文件类型/文献管理软件名称

选择包含的内容

泌盐植物长叶红砂质膜Na⁺/H⁺逆向转运蛋白基因(RtSOS1)全长cDNA的克隆及序列分析

Cloning and Sequence Analysis of the Plasma Membrane Na⁺/H⁺Antiporter cDNA in Recretohalophyte Reaumuria trigyna Maxim.

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

泌盐植物长叶红砂质膜Na+/H+逆向转运蛋白基因(RtSOS1)全长cDNA的克隆及序列分析

Cloning and Sequence Analysis of the Plasma Membrane Na+/H+Antiporter cDNA in Recretohalophyte Reaumuria trigyna Maxim.

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价

泌盐植物长叶红砂质膜Na⁺/H⁺逆向转运蛋白基因(RtSOS1)全长cDNA的克隆及序列分析

Cloning and Sequence Analysis of the Plasma Membrane Na⁺/H⁺Antiporter cDNA in Recretohalophyte Reaumuria trigyna Maxim.