华北农学报 ›› 2012, Vol. 27 ›› Issue (1): 98-101. doi: 10.3969/j.issn.1000-7091.2012.01.018

所属专题: 油料作物

• 论文 • 上一篇    下一篇

甘蓝型油菜白花性状的遗传及AFLP标记

黄镇, 许婷, 班元元, 刘欢, 范胜栩, 杨丽, 徐爱遐   

  1. 西北农林科技大学农学院, 陕西杨凌 712100
  • 收稿日期:2011-08-12 出版日期:2012-02-28
  • 通讯作者: 徐爱遐(1964- ), 女, 陕西三原人, 副教授, 博士, 主要从事油菜遗传育种研究。
  • 作者简介:黄镇(1981- ), 男, 湖北武汉人, 讲师, 博士, 主要从事油菜遗传育种及分子生物学研究。
  • 基金资助:
    西北农林科技大学唐仲英育种基金(A212020905;A212020523);国家"863"项目(2009AA101105)

Genetic Studies of White Petals and AFLP Markers Linked to White Petal Gene in Brassica napus L.

HUANG Zhen, XU Ting, BAN Yuan-yuan, LIU Huan, FAN Sheng-xu, YANG Li, XU Ai-xia   

  1. College of Agronomy, Northwest Agriculture and Forestry University, Yangling 712100, China
  • Received:2011-08-12 Published:2012-02-28

摘要: 甘蓝型油菜的白花性状是一种有用的指示性状,本研究以甘蓝型白花油菜及其分离群体为材料,对甘蓝型油菜的白花性状的遗传规律进行了研究。结果表明,甘蓝型油菜的白花性状受1对不完全显性基因控制。利用集团分离法(BSA) 对白花基因进行AFLP分析,在256对AFLP引物中共获得6个与白花基因紧密连锁的分子标记。EA11MC04与EA12MC01为基因两侧最近的分子标记,其遗传距离分别为0.8,1.0 cM。

关键词: 甘蓝型油菜, 白花, 遗传, 分子标记

Abstract: White character is a useful marker trait in Brassica napus L, white petal rapeseed and their segregation populations were used as experiment materials to study the inheritance of white petal traits. The results showed that the white petal color character was controlled by one pair of incomplete dominant gene. Bulked segregation analysis(BSA) was employed to identify the amplified fragment length polymorphism(AFLP) markers linked to the white petal gene, from a survey of 256 AFLP primer combinations, six AFLP markers tightly linked to the white petal gene were identified. In which EA11MC04 and EA12MC01, the two closest markers, were located on either side of the white petal gene at a distance of 0. 8 and 1. 0 cM, respectively.

Key words: Brassica napus L., White petal, Genetics, Molecular markers

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引用本文

黄镇, 许婷, 班元元, 刘欢, 范胜栩, 杨丽, 徐爱遐. 甘蓝型油菜白花性状的遗传及AFLP标记[J]. 华北农学报, 2012, 27(1): 98-101. doi: 10.3969/j.issn.1000-7091.2012.01.018.

HUANG Zhen, XU Ting, BAN Yuan-yuan, LIU Huan, FAN Sheng-xu, YANG Li, XU Ai-xia. Genetic Studies of White Petals and AFLP Markers Linked to White Petal Gene in Brassica napus L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(1): 98-101. doi: 10.3969/j.issn.1000-7091.2012.01.018.

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