摘要: 1984、1985年的试验结果,明确了温度、紫外光、日光及缓冲液pH值等几种物理因素对山楂粉蝶核型多角体病毒Acr NPV感染力的影响.Acr NPV分别在40℃冰箱中保存14个月、28个月和在果园树干上保留14个月的Acr NPV虫尸,对幼虫的感染力无明显变化.Acr NPV存32℃恒温处理后,其感染力随时间延长而逐渐下降.紫外光对Acr NPV杀伤力较大,当紫外光照射240分钟后,Acr NPV完全丧失了对幼虫的感染力.果园试验表明Acr NPV悬液喷在果树叶正面和背面时,对幼虫的感染力有差异.Acr NPV对幼虫的感染力和磷酸缓冲液的PH值有关,当pH值为3.5或10.5时,感病幼虫发病慢,当pH值为7.2时,幼虫发病快.
关键词:
山楂粉蝶核型多角体病毒,
感染力,
物理因素
Abstract: The experiments in 1984 and 1985 showed that physical factors such as temperature ultraviolet light sunlight and pH of the buffer can influence the infectious ability of the Acr NPV.The infectious ability of the Acr NPV does not vary whether it is stored in a refrigerator at 4? for 14 months and 28 months or in an infected body that stayed on a tree trunk in an orchard for 14 months.The infectious ability of the Acr NPV treated at 35? decreases in relation to the length of treatment. The Acr NPV can be damaged seriously by ultraviolet light and lost its infection ability entirely when it is irradiated for 240 minutes with ultraviolet rays.Experiments in the orchard show that the infectious ability is different when the Acr NPV suspension is sprayed on the faces and the backs of the leaves.The infectious ability of the Acr NPV is related to the pH of a phospha-tic buffer. The infected larvae fall ill slowly when the pH is at 3. 5 or at 10-5 and fall fast when at 7. 2.
Key words:
Acr NPV (Nuclear Polynedrosis Virus of Aporia crataegi),
Infectious ability,
Physical factors influence
李岩涛. 山楂粉蝶核型多角体病毒研究[J]. 华北农学报, 1987, 2(2): 63-68. doi: 10.3321/j.issn:1000-7091.1987.02.010.
Li Yantao. A STUDY ON NUCLEAR POLYNBDROSIS VIRUS OF APORIA CRATAEGI[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1987, 2(2): 63-68. doi: 10.3321/j.issn:1000-7091.1987.02.010.