芥蓝的研究多集中在分类、常规种资源遗传多样性等方面。随着芥蓝杂交种的问世，杂交种的优势越来越突出，市场效果明显，因此，对芥蓝自交系的遗传多样性等研究尤为重要。选取分布在甘蓝9条染色体上的55对SSR引物，从中筛选出多态性好、分布均匀的15条SSR引物对98份芥蓝自交系进行分析，共得到65个多态性带型。采用UPGMA方法构建了一张聚类图，在相似系数为0.71时，将98份芥蓝自交系初步分为1，2，3，4，5组。研究结果为芥蓝育种中的亲本选配、杂交种鉴定和资源保护等提供了依据。在试配芥蓝新组合时，应选择遗传距离较远的自交系作为重点材料，后代的杂种优势会更显著。
张德双
,
张娜
,
于拴仓
,
张凤兰
,
隋光磊
,
余阳俊
,
赵岫云
,
汪维红
,
苏同兵
,
卢桂香
. 基于SSR分子标记的芥蓝遗传多样性分析[J]. 华北农学报, 2014
, 29(4)
: 140
-144
.
DOI: 10.7668/hbnxb.2014.04.024
Classification and genetic diversity of local varieties in Chinese kale were studied before.With the extending of Chinese kale hybrids,they can show higher heterosis and better benefit.Therefore study on genetic diversity of inbred lines is important now.In this research,98 Chinese kale inbred lines were analyzed by 15 polymorphic SSR markers which were selected from 55 pairs of primers locating on 9 chromosomes of cabbage.65 polymorphic bands were detected by these 15 pairs of primers.UPGMA method was used to determine genetic diversities of 98 Chinese kale inbred lines.They were initially clustered intoⅠ,Ⅱ,Ⅲ,Ⅳ,Ⅴ groups with coefficient 0.71.Results can be used to breed Chinese kale including selection and match of parents,identification of hybrids and protection of resources.
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