为了提高牛卵母细胞体外成熟率，以机械裸卵（DOs）、卵丘-卵母细胞复合体（COCs）和添加的离散卵丘细胞的不同组合，对卵丘细胞在卵母细胞体外成熟过程中的影响进行了探索，并对不同卵丘细胞的作用途径和强度进行了分析。试验结果表明：添加离散卵丘细胞能够显著促进DOs的体外成熟（57.98±14.27 vs 35.53±14.00，P<0.05），对COCs具有促进趋势但差异不显著（76.66±5.77 vs 66.76±9.46，P>0.05）；卵母细胞胞外卵丘细胞对与之共培养的DOs的体外成熟促进作用效果不明显（35.83±18.32 vs 35.53±14.00，P>0.05）。分析结果显示：卵母细胞胞外卵丘细胞主要通过间隙连接促进卵母细胞的体外成熟，添加的离散卵丘细胞主要以旁分泌途径促进卵母细胞的体外成熟；3层及其以上胞外卵丘细胞能够提高其包裹的卵母细胞平均体外成熟能力的85.34%，添加的离散卵丘细胞能够提高COC平均体外成熟能力的16.18%，能够提高DO平均体外成熟能力的60.61%。
To improve the in vitro maturation rate of bovine oocytes,the present study was carried out to determine the effects of cumulus cell on in vitro maturation rate of cumulus-oocytes-complexs(COCS)and artificial denuded oocytes(DOs),and its pathway and strength was also investigated.The results showed that co-culture with discrete cumulus cells significantly improved the in vitro maturation rate of denudes oocytes(57.98±14.27 vs 35.53± 14.00,P<0.05),but had no effects on COCS(76.66±5.77 vs 66.76±9.46,P>0.05);Co-culture with surrounding cumulus cell did not significantly improve the in vitro maturation rate of denudes oocytes(35.83±18.32 vs 35.53±14.00,P>0.05).The analysis results showed that cumulus cell promoted the in vitro maturation of oocytes by the pathway of gap-junction,however discrete cumulus cells worked by the pathway of paracrine;Three or more layers cumulus improved the mean maturation ability of their surrounded oocytes by 85.34%,added discrete cumulus cells separately improved the mean maturation ability of COCs and DOs by 16.18% and 60.61%.
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