论文

Y系山定子中花发育相关基因MADS5的克隆与功能分析

  • 樊新萍 ,
  • 乔永胜 ,
  • 杨廷帧 ,
  • 王骞 ,
  • 石美娟 ,
  • 蔡华成 ,
  • 高敬东 ,
  • 牛西午
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  • 1. 山西大学 生命科学院, 山西 太原 030006;
    2. 山西省农业科学院 果树研究所, 山西 太谷 030800;
    3. 山西省农业科学院, 山西 太原 030006
樊新萍(1973-),女,山西永济人,副研究员,硕士,主要从事果树分子生物学研究。

收稿日期: 2014-02-17

  网络出版日期: 2014-09-20

基金资助

山西省国际科技合作项目(2012081002)

Clone and Function Analysis of MADS5 Gene Related to Flower Development of Y Series Dwarf Apple Stock

  • FAN Xin-ping ,
  • QIAO Yong-sheng ,
  • YANG Ting-zhen ,
  • WANG Qian ,
  • SHI Mei-juan ,
  • CAI Hua-cheng ,
  • GAO Jing-dong ,
  • NIU Xi-wu
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  • 1. College of Life Science, Shanxi University, Taiyuan 030006, China;
    2. Pomology Institute, Shanxi Academy of Agricultural Sciences, Taigu 030800, China;
    3. Shanxi Academy of Agricultural Sciences, Taiyuan 030006, China

Received date: 2014-02-17

  Online published: 2014-09-20

摘要

以Y系山定子为中间砧嫁接的苹果树当年开始花芽形态分化,而未用中间砧的苹果树则未形成花芽。分析了中间砧Y系山定子与基砧海棠不同组织中花发育相关基因 MADS5MADS2MdFT2MdAFL1MdAFL2MdTFL1 的表达特征。结果表明,MADS5 基因的表达模式及表达量是影响苹果花芽发育的一个重要因素;将山定子中的 MADS5 基因在拟南芥中过量表达,转基因拟南芥表现出早花表型,比对照早开花7~10 d;相同条件下转 MdFT2 基因的拟南芥比对照早开花4~7 d。认为在利用基因工程改良苹果早花发育的研究中,MADS5 基因更具有应用潜力。

本文引用格式

樊新萍 , 乔永胜 , 杨廷帧 , 王骞 , 石美娟 , 蔡华成 , 高敬东 , 牛西午 . Y系山定子中花发育相关基因MADS5的克隆与功能分析[J]. 华北农学报, 2014 , 29(4) : 25 -31 . DOI: 10.7668/hbnxb.2014.04.005

Abstract

The flower bud formation of apple trees grafted into Y series dwarf apple interstock was earlier than those not grafted into the Y series interstock.In this study,we analyzed the expression pattern of several flower development genes,including MADS5, MADS2, MdFT2, MdAFL1, MdAFL2, MdTFL1, in the different tissues of Y series dwarf apple interstock(Malus baccata)and rootstock(Malus asiatica).The results showed that MADS5 gene was a key regulator in control of apple bud formation due to its special expression pattern and higher expression level in bud and sepal tissues.Overexpression of Y series dwarf apple interstock MADS5 gene in Arabidopsis reduced the vegetative growth and generation time and led to an early blooming phenotype.Transgenic plants overexpression MADS5 gene blossomed earlier about 7-10 days compared with the wild type plants.Meanwhile,overexpression of MdFT2 gene in Arabidopsis also promoted flowering average 4-7 days earlier compared with the wild type plants.It is concluded that MADS5 gene is more useful than MdFT2 gene in the study of early blooming promoting and fruiting of apple plants through genetic engineering methods.

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