利用水稻稻瘟病抗病基因Pi-b、Pi-ta、Pikm和 Pi54 的功能标记检测2012年江苏省迟熟中粳稻预试64份品系，结合水稻穗颈瘟抗性鉴定，解析稻瘟病抗病基因Pi-b、Pi-ta、Pikm和 Pi54 在江苏省粳稻稻瘟病抗性育种中的作用。结果表明，在64份材料中含稻瘟病基因Pi-b的有49份，含Pi-ta的26份，含Pikm的19份，含 Pi54 的25份。64份品系的人工或病圃鉴定其抗性表现为2级中抗的有10份，3级感病的有43份，4级高感的有11份。64份品系中含有抗稻瘟病基因Pi-ta(26个新品系)，或Pi-b、Pikm和 Pi54 组合(4个新品系)，则该品系穗颈瘟抗性水平在2级中抗或3级感病，无4级高感，其他抗病基因组合其穗颈瘟抗性水平一般都存在4级高感。相关性分析表明，只有抗稻瘟病基因Pi-ta与穗颈瘟抗性存在显著相关性(r=0.344，P<0.01)。
范方军
,
王芳权
,
刘永峰
,
王军
,
朱金燕
,
李文奇
,
仲维功
,
杨杰
. Pi-b、Pi-ta、Pikm和Pi54对水稻穗颈瘟的抗性评价[J]. 华北农学报, 2014
, 29(3)
: 221
-226
.
DOI: 10.7668/hbnxb.2014.03.040
In this study, 64 late maturity japonica test lines of Jiangsu Province were detected with the functional markers of rice blast resistance genes Pi-b, Pi-ta, Pikm and Pi54 in rice.Combined with the identification of rice panicle blast resistance, the role of blast resistance genes Pi-b, Pi-ta, Pikm and Pi54 for Japonica rice breeding with blast resistance in Jangsu Province has been analysized.There were 49 lines containing rice blast resistance gene Pi-b, 26 lines containing Pi-ta, 19 lines containing Pikm, 25 lines containing Pi54 in 64 lines.Through artificial or disease nursery identification, the resistance performance into 2 levels(Moderate resistance) of disease resistance were 10 lines, 3 levels(Susceptible) were 43 lines, 4 levels(High susceptible) were 11 lines.If new lines contain blast resistance genes Pi-ta(26 new lines), or combined with Pi-b, Pikm and Pi54 (4 new lines), the levels of panicle blast resistance showed 2 levels(Moderate resistance) or 3 levels(Susceptible) and never 4 levels(High susceptible), while lines with other resistance gene combinations generally exhibited 4 grade levels of panicle blast resistance(High susceptible).A correlation showed that only the rice blast resistance gene Pi-ta has certain correlation with Panicle Blast Resistance(r=0.344, P<0.01).
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