Chrysanthemum Jinba was used as material to study the influence of exogenous spermidine(Spd) and polyamine inhibitors D-Arginine(D-Arg) in roots and leaves on polyamine oxidase(PAO), diamine oxidase(DAO), peroxidase(POD), catalase(CAT), hydrogen peroxide(H2O2) and lignin content of flower bud differentiation stage.Results showed that, the order of PAO, DAO activity and lignin content in root and leaf were Spd>CK>D-Arg.Exogenous Spd significantly promoted polyamine oxidases activity and the synthesis of lignin;D-Arg reduced the polyamine oxidases activity and the synthesis of lignin and inhibit the flower bud differentiation.The results showed that under the conditions of short day, polyamine may affect synthesis of lignin by influencing activity of polyamine oxidases and its reaction production H2O2, thereby regulate flower bud differentiation of chrysanthemum.
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