为了筛选P1病毒VP1蛋白的特异性单抗，通过生物信息学软件预测其抗原表位，然后利用SEALTM方法制备针对不同抗原表位的单抗，再通过免疫组化试验和Western Blot对其中6株进行分析，筛选敏感性强、特异性好的单抗。结果显示，选取的6株单抗中有3 株(mAb1、mAb6和mAb9)在免疫组化染色时呈阳性反应，有1株(mAb1)在Western Blot 中表现强阳性。研究筛选出了P1 VP1蛋白的特异性单抗，为P1病毒的检测和疫苗研究奠定了基础。
To select specific mAbs for VP1 protein of P1 virus, epitopes of it were predicted by bioinformatics software, and then mAbs against different epitopes were prepared by the method of SEALTM and applied to immunohistochemistry and Western Blot analysis, respectively, for screening sensitive and specific mAbs.Results showed that there were three positive mAbs(mAb1, mAb6 and mAb9) in immunohistochemical staining and one mAb(mAb1) showed strong hyacinthine in Western Blot.In a nutshell, specific mAbs were screened for VP1 protein of P1 virus, which will contribute to the detection of P1 and its vaccine development.
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