论文

叶霉菌粗毒素对番茄幼苗防御酶及活性氧的诱导

  • 刘慧芹 ,
  • 刘慧平 ,
  • 韩巨才
展开
  • 1. 天津农学院, 园艺系,天津 300384;
    2. 山西农业大学, 农学院,山西 太谷 030801
刘慧芹(1973-),女,山西太原人,讲师,硕士,主要从事生物病理学研究.

收稿日期: 2009-09-22

  网络出版日期: 2014-10-14

基金资助

山西省留学归国基金项目(2009043;2007061);山西省科技攻关项目(2007031039)

Induce of Crude Toxin from Cladosporium fulvum on Defense Enzyme and Activity Oxygen of Tomato Seedlings

  • LIU Hui-qin ,
  • LIU Hui-ping ,
  • HAN Ju-cai
Expand
  • 1. Department of Horticulture, Tianjin Agricultural University, Tianjin 300384, China;
    2. Agronomy College, Shanxi Agricultural University, Taigu 030801, China

Received date: 2009-09-22

  Online published: 2014-10-14

摘要

以番茄不同抗性品种的幼苗为材料,采用浸渍法研究了低浓度的叶霉菌粗毒素对番茄叶片防御酶(SOD 、POD 、PAL)活性及活性氧(O2-和H2O2)的诱导作用,为探讨病菌毒素诱导植物抗病性的生理生化机制提供科学依据.结果表明,番茄经叶霉菌粗毒素诱导后,抗、感品种叶片的SOD活性下降,但抗病品种的下降比率小,且比感病品种具有较高的酶活性;抗、感品种的POD活性变化趋势均为先上升后下降,二者间的酶活性变化差异不大; 抗、感品种PAL活性均升高,但抗病品种增加更快,且增加比率高于感病品种;抗、感品种的O2-产率和H2O2含量的变化呈增加趋势,抗病品种的增加比率均高于感病品种,且增加峰值出现早.说明叶霉菌粗毒素诱导的番茄体内防御酶和活性氧变化与番茄抗叶霉病有着密切的关系.

本文引用格式

刘慧芹 , 刘慧平 , 韩巨才 . 叶霉菌粗毒素对番茄幼苗防御酶及活性氧的诱导[J]. 华北农学报, 2009 , 24(6) : 215 -218 . DOI: 10.7668/hbnxb.2009.06.044

Abstract

The inducement of crude toxin from Cladosporiumfulvum on defense enzyme (SOD,POD,PAL) and ac tivity (O2-,H2O2) was studied in leaves of different tomato varieties by soaking method.The studies provided scientific basis on plant physiological and biochemical resistance mechanisms induced by pathogentoxin.The results showed that superoxide dismutase (SOD) activity of all varieties descended,but the resistant variety had lower declining ratio and higher enzyme activity than that of the susceptible one.Changes of peroxidase (POD) activity in two varieties were simi lar.POD activity increased at first,followed by a decline,but two varieties had a little diversity in activity change.Pheny lalanine ammonialyase (PAL) activity increased in two varieties,but PAL activity in the resistant variety increased fast,and increasing ratio was higher than that in susceptible one.Production rate of superoxide anion (O-) and hydrogen peroxide (H2O2) content increased.Increasing ratio in resistant variety was higher than that in susceptible one.The ac tivity in the resistant variety appeared earlier than that in the susceptible one.Changes of defense enzyme and activity oxygen induced by toxin from Cladosporium fulvum in tomato seedlings were closely related to their resistance.

参考文献

[1] 王金生.分子植物病理学[M].北京:中国农业出版社,2001.
[2] Smith C J.Biochemistry and molecular biology of plant pathology interaction[M].Oxford:Clarendon Press,1991:255-270.
[3] Heil M,Bostock R M.Induced systemic resistance (ISR) against pathogens in the context of induced plant defences[J].Annals of Botany,2002,89:503-512.
[4] 张树生,胡蕾,刘忠良,等.植物体内抗病相关酶与植物抗病性的关系[J].安徽农学通报,2006,12(13):48-49.
[5] 邱金龙,王钧,余明珍.活性氧与植物抗病反应[J].植物生理学通讯,1998,34(1):56-63.
[6] Ingeborg T,Christiane L,Alok K S.Cyclopentenone isoprostanes induced by reactive oxygen species trigger defense gene activation and phytoalexin accumulation in Plants[J].The Plant J,2003,34(3):363-368.
[7] Lazarovits G,Higgins V J.Biological activity and specificity of a toxin produced by Cladosporium fulvum[J].Physiology and Biochemistry,1979,69(10):1056-1061.
[8] Peever T L,Higgins V J.Electrolyte leakage,lipoxygenase,and lipid peroxidation induced in tomato leaf tissue by specific and nonspecific elicitors from Cladosporium fulvum[J].Plant Physiol,1989,90:867-875.
[9] De Wit P J,Laug R,Hon e G,et al.Molecular and biochemical basis of the interaction between tomato and its fungal pathogen Cladosporium fulvum[J].Antonie van Leeuwenhoek,1997,71:137-141.
[10] 刘慧芹,刘慧平,韩巨才.叶霉病菌粗毒素对番茄的生物活性测定[J].天津农学院学报,2004,11(1):9-11.
[11] 高俊凤.植物生理学实验技术[M].西安:世界图书出版社,2000.
[12] 王爱国,罗光华.植物的超氧自由基与羟反应的定量关系[J].植物生理学通讯,1990,26(6):55-57.
[13] Patterson B D,Mackae E A,Fergusen I B.Estimation of hydrogen peroxide in plant extracts using titanium (IV)[J].Anal Biochem,1984,39:487-492.
[14] 梁建根,张炳欣,陈振宇,等.促生菌CH1诱导黄瓜对猝倒病抗性的研究[J].园艺学报,2006,33(2):283-288.
[15] 李海莲,侯喜林,易金鑫,等.黄萎病菌粗毒素接种对感病和抗病茄子品种的一些酶类活性和光合特性的影响[J].植物生理学通讯,2005,41(4):453-456.
[16] 崔彦玲,张环.番茄叶霉病与苯丙氨酸解氨酶的相关性[J].华北农学报,2003,18(1):79-82.
[17] 潘哲超,陈建斌,范静华,等.稻瘟菌粗毒素对水稻防御性相关酶系的诱导[J].云南农业大学学报,2008,23(2):162-166.
[18] Wojtaszek P.Oxidative burst:an early plant response to pathogen infection[J].Biochem J,1997,322(3):681-692.
[19] Lamb C,Dixon R A.The oxidative burst in plant disease resistance[J].Ann Rev Plant Physiol,1997,48:251-275.
文章导航

/