黄瓜枯萎病是造成黄瓜减产的主要病害之一,研究黄瓜枯萎病抗性分子机理对黄瓜抗病育种有重要意义.以黄瓜抗枯萎病材料Cu1为试材,采用基因差异显示技术(DDRT-PCR)研究黄瓜接种枯萎病生理小种后基因的表达情况,分离出1个接种后在根茎部特异表达的差异cDNA片断,命名为A178-2.A178-2基因片段的328个核苷酸中有15个核苷酸与拟南芥泛素蛋白同源,氨基酸序列同源性达到85%.
沈凤瑞, 吴萍, 毛爱军, 周红梅, 王永健, 于拴仓, 张丽蓉, 杨艳丽, 郑晓鹰
. 黄瓜抗枯萎病相关基因cDNA的分离及鉴定[J]. 华北农学报, 2010
, 25(1)
: 40
-43
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DOI: 10.7668/hbnxb.2010.01.008
Fusarium wilt(Fusarium oxysporum f.sp.cucumerinum Owen.) is one of major diseases that cause significant disease losses in cucumber.It is significant to explore the disease resistance molecular mechanism against Fusarium wilt race 4 in cucumber breeding.The cucumber resistant line Cu14 were employed for studying on resistant genes expressing against Fusarium wilt by differential display reverse transcriptase PCR(DDRT-PCR) method.One fragment of cDNA expressed specially was got from the root-stem inoculated in cucumber,and was named as A178-2 with the size of 328 bp.Blast analysis showed that 145 bp nucleotide sequences of A178-2 was homologous with the generic grain protein in Arabidopsis and reached 85%.Further studying on expression of the gene will be done.
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