在大田条件下,研究了追氮时期对两种穗型冬小麦品种氮素的积累、运转以及蛋白质组分的影响.结果表明,拔节期追氮显著增加开花期和成熟期小麦植株氮素积累量及转运量,而抽穗期追氮反而下降,氮素转运率和贡献率则随追氮时期后移而降低.兰考矮早八GS活性和豫麦49-198 NR活性在所有测定日期均表现随追氮时期后移而增加,兰考矮早八NR和豫麦49-198的GS活性在灌浆前期呈现与此相同趋势,而在灌浆后期则以拔节期追氮最高.推迟追氮时期,两品种籽粒总蛋白、清蛋白、醇溶蛋白和谷蛋白含量均增加,而球蛋白却降低.兰考矮早八剩余蛋白含量随追氮时期后移而降低,豫麦49-198则相反.
The field experiment was carried out to investigate the effects of nitrogen topdressing stage on nitrogen accumulation,transfer,assimilation enzymes and protein compontents in two wheat cultivars with different spike types.The results indicated that nitrogen topdressing at jointing stage increased N accumulation at anthesis and maturity and N transfer compared with nitrogen topdressing at turn green stage and heading.N translocation efficiency and N contribution proportion decreased with nitrogen topdressing stage delaying.GS activity of flag leaves in Lankaoaizao8 and NR in Yumai 492198 increased with the nitrogen topdressing stage delaying,and NR activity of flag leaves in Lankaoaizao8 and GS in Yumai 492198 took on above change rules in early filling with maximum of nitrogen topdressing at jointing stage in late filling.Albumin,gliadin,glutenin and total protein content increased with the nitrogen topdressing stage delaying,and globulin content decreased.The other protent content in Lankaoaizao8 decreased with the nitrogen topdressing stage delaying,and increased in Yumai 492198.
[1] 王月福,于振文,李尚霞,等.氮素营养水平对冬小麦氮代谢关键酶活性变化和籽粒蛋白质含量的影响[J].作物学报,2002,28(6):743-748.
[2] 李泽松,张楚福,林清华,等.不同氮源对水稻幼苗根氨同化酶的影响[J].武汉大学学报:自然科学版,2000,46(6):729-732.
[3] 张庆江,张立言,毕桓武.普通小麦碳氮物质积累分配特征及与籽粒蛋白质的关系[J].华北农学报,1996,11(3):57-62.
[4] 张庆江,张立言,毕桓武.春小麦品种氮的吸收积累和转运特征及籽粒蛋白质含量关系[J].作物学报,1997,23(6):712-718.
[5] Dechard E L.Nitrate reductase assays as prediction teat for crosses and lines in spring wheat[J].Crop Science,1978,18:289-294.
[6] 王小纯,熊淑萍,马新明,等.不同形态氮素对专用型小麦氮代谢关键酶活性及籽粒蛋白质含量的影响[J].生态学报,2005,25(4):802-808.
[7] 李永庚,蒋高明,杨景成.温度对小麦碳氮代谢、产量及品质影响[J].植物生态学报,2003,27(2):164-169.
[8] 蒋家慧,隋春青,衣先众.氮肥运筹对小麦氮素同化、运转和品质的影响[J].莱阳农学院学报,2004,21(3):217-219.
[9] 沈建辉,戴廷波,荆奇,等.施氮时期对专用小麦干物质和氮素积累、运转及产量和蛋白质含量的影响[J].麦类作物学报,2004,24(1):55-589.
[10] 荆奇,戴廷波,姜东,等.不同生态条件下不同基因型小麦干物质和氮素积累与分配特征[J].南京农业大学学报,2004,27(1):1-5.
[11] 王月福,杜金哲,梁作正,等.不同种小麦氮素同化、运转和分配的比较研究[J].莱阳农学院学报:自然科学版,2006,23(3):191-195.
[12] 刘晓冰,李文雄,周鹏.春小麦产量和蛋白质关系研究-干物质积累分配与氮素同化运转[J].东北农业大学学报,1996,27(2):116-123.
[13] 中国科学院上海植物生理研究所.现代植物生理学实验指南[M].北京:科学出版社,1999.
[14] 杨文钰,韩惠芳,任万君,等.烯效唑干拌种对小麦分蘖期间内源激素及糖氮比的影响[J].作物学报,2005,31(6):760-765.
[15] Heitholt J J,Croy L I,Maness N O,et al.Nitrogen partitioning in genotypes of winter wheat differing in grain N concentration[J].Field Crops Research,1990,23:133-144.
[16] Johnson J W,Hargrove W L,Touchton J T,et al.Influence of N fertilization on wheat milling and baking quality[J].Crop Science,1984,24:904-906.
[17] 田纪春,张忠义,梁作勤.高蛋白质和低蛋白质小麦品种氮素吸收和运转分配差异的研究[J].作物学报,1994,20(1):76-83.
[18] 马冬云,郭天财,查菲娜,等.种植密度对两种穗型冬小麦旗叶氮代谢酶活性及籽粒蛋白质含量的影响[J].作物学报,2007,33(3):514-517.
[19] 林清华,李常键,彭进,等.NaCl对水稻谷氨酸合酶和谷氨酸脱氢酶的胁迫作用[J].武汉植物学研究,2000,18(3):206-210.
[20] Dalling M J,Boland G,Willson J H.Relation between acid proteinase activity and redistribution of nitrogen during grain development in wheat[J].Australian Journal of Plant Physiology,1976,3:721-730.
[21] Crosbic G B,Ross A S,Morot T,et al.Starch and protein quality requirement of Japanese alkaline noodles (Ramen)[J].Cereal Chemistry,1999,76(3):328-334.
[22] Hou G.Oriental noodles[J].Advances in Food and Nutrition Research,2001,43:143-193.
[23] Baik B K,Czuchajowska Z,Pomeranz Y.Role and contribution of starch and protein contents and quality to texture profile analysis of oriental noodles[J].Cereal Chemistry,1994,71:315-320.
[24] Yuns H,Quail K J,Moss R.Physicochemical properties of Australian wheat flours for white salted noodles[J].Journal of Cereal Science,1996,23:181-189.
[25] 王珏,杜金哲,胡尚连,等.春小麦不同品质类型籽粒蛋白质及组分与沉淀值的关系[J].莱阳农学院学报,2004,21(3):227-230.
[26] 李志西,魏益民,张建国,等.小麦蛋白质组分与面团特性和烘焙品质关系的研究[J].中国粮油学报,1998,13(3):1-5.
[27] 王月福,于振文,李尚霞,等.施氮量对小麦籽粒蛋白质组分含量及加工品质的影响[J].中国农业科学,2002,35(9):1071-1078.
