为探讨鉴别山羊痘病毒和绵羊痘病毒的分子生物学方法,对实验室分离保存的3株山羊痘病毒和2株绵羊痘病毒进行p32基因和GpCR基因扩增、克隆及序列分析.将获得的p32基因序列与GenBank上登陆的羊痘病毒p32基因进行HinfⅠ酶切位点分析表明,所有绵羊痘病毒p32基因在391 bp和691 bp处存在2处酶切位点,而山羊痘病毒仅在688 bp处存在1个酶切位点.将获得的GpCR基因序列与GenBank上登录的31条相应序列进行系统进化树分析发现,根据GpCR基因序列信息可将绵羊痘病毒和山羊痘病毒分成两个独立的进化分枝.这些结果表明,利用p32基因的HinfⅠ酶切位点信息和对GpCR基因进行分子进化分析可鉴别山羊痘病毒和绵羊痘病毒,p32基因和GpCR基因可作为鉴别绵羊痘病毒和山羊痘病毒的特异性标记基因.
To evaluate the methodology for Discrimination of goatpoxvirus (GPV)and sheeppoxvirus (SPV),the p3genes and G 2protein2coupled chemokine receptor (GpCR) genes amplified from 3 strains of SPV and strains of GPV which isolated from the field samples were sequenced and compared with the corresponding sequences deposited in GenBank.Comparison of Hinf Ⅰrestriction enzyme sites of p3genes showed that there are sites located in 391 bp and 691 bp among all the SPV strains,and 1 site in 688 bp among GPV strains,respectively.Cladogram generated by sequence comparison with GpCR genes showed that the SPV and GPV belongs to the two different branches.The results indicated that the both genes,p3and GpCR,can be the candidates for development of methodologies for discrimination of GPV and SPV.
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