对加拿大披碱草、老芒麦、杂种F1、CoⅠ、C1Ⅰ、CoⅡ和C1Ⅱ7种材料进行了同工酶和RAPD的分析,旨在通过分子标记方法探讨供试材料的亲缘关系.结果表明:在不同的生育期,EST同工酶酶谱具有较高的多态性,酶谱差异较大,在分蘖期杂种的EST酶谱偏向母本加拿大披碱草,根据该生育时期的酶谱特征可以鉴定杂种后代的的真实性,其他生育时期,EST酶带偏向父本老芒麦,而且没有明显的差异.SOD酶谱的差异只是体现在表达量的多少上,并没有出现特异性的条带.RAPD分析表明,加拿大披碱草与老芒麦的遗传一致度为0.72,遗传距离为0.32 5,在7种材料中亲缘关系最远;杂种后代与母本加拿大披碱草的遗传距离较近,而与父本老芒麦的亲缘关系相对较远;因此用RAPD分析材料的遗传关系更为合适.
Genetic analysis on isozymes and RAPD of E.canadensis,E.sibiricus,F1、CoⅠ、C1Ⅰ、CoⅡ和C1Ⅱ were done.The results showed that the polymorphism of EST isozymes were much more than that of SOD during the four breeding periods.At tillering stage,the results of esterase isozymes also showed that true hybrid could be identificate,and zymogram characteristics of esterase isozymes tended to that of female parent E.canadensis.At other three breeding periods,zymogram characteristics of esterase isozymes tended to that of male parent and there were little differences.Differences of SOD isozyme only lied in expression level,and there were no specific band.Analysis on RAPD showed that genetic identity and genetic distance of E.canadensis and E.sibiricus were 0.7and 0.328 5.Their genetic relationship was most furthest among 7 materials.And genetic relationship between hybrids and parents showed that hybrids tended to female parent E.canadensis.But that was very far to male E.sibiricus.It was much appropriate that RAPD was used to analyze genetic relationship.
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