腐殖质呼吸是一种新型微生物能量代谢方式,菌种资源研究成为腐殖质呼吸的研究重点。利用厌氧富集分离方法,从木薯堆肥土壤中获得1株具有腐殖质还原特性的兼性厌氧菌株,编号为HN02。HN02为革兰氏阳性菌,细胞为短杆状,菌落呈黄色,表面光滑,边缘整齐;最适生长盐度为1%,最适生长温度为30℃,最适生长pH值7.5～8.5;可利用麦芽糖作为碳源,明胶液化和硝酸盐还原为阴性;16S rDNA基因序列分析结果表明:菌株HN02与天花板节杆菌亲缘关系最近,同源性达99.4%;HN02能在以葡萄糖作为电子供体,以AQDS为唯一电子受体的厌氧环境下将AQDS还原为AH2QDS,还原率达40%。菌株HN02是1株具有腐殖质还原特性的天花板节杆菌,首次报道该菌属菌株具有腐殖质还原特性,为完善腐殖质还原特性的微生物系统提供理论基础。
The humus respiration is a new way of microbial energy metabolism．And the research about strains resources becomes the focus point．A facultative anaerobic humus- reducing bacterium，designated HN02，was isola- ted from cassava dregs composting．Strain HN02 is a gram- positive，short rod- shaped，gelatin hydrolysis and nitrate reduction- negative bacterium．Optimal growth salt was 1% (NaCl); optimal growth temperature was 30 ℃ and opti- mal pH was at 7． 5－ 8． 5． Analysis of 16S rDNA of HN02 indicated that the closest phylogenetic relative among the valid species was Arthrobacter tecti，with 99． 4% 16S rDNA similarity．Strain HN02 can utilize AQDS via anaerobic enrichment procedure with glucose as the electron donor and AQDS as the sole terminal electron acceptor．The re- duction ratio of AQDS was 40%．Thus，the humus- reducing of Arthrobacter tecti HN02 is here reported for the first time and it can provides the theoretical basis for microbial systems with characteristics of humus reduction．
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