为了研究不同猪种(山东省地方猪种莱芜黑猪和引进猪种大约克)和FUT I不同基因型仔猪对肠毒性大肠杆菌F18(ETEC F18)的抵抗能力和肠黏膜上皮细胞对ETEC F18黏附能力的差异,在采用PCR-RFLP技术对莱芜黑猪和大约克FUT I基因型检测的基础上,选取易感性和抗性断奶仔猪,利用野生型ETEC F18标准株进行试验猪口服细菌攻毒试验和肠黏膜黏附试验。由于多方面原因,接受ETEC F18细菌攻毒的试验猪均未发病,无法判断试验猪对ETEC F18的抵抗能力。ETEC F18标准菌株能与所有FUT I敏感基因型仔猪的小肠黏膜上皮细胞黏附,而不能与抗性基因型仔猪小肠上皮黏膜细胞黏附,莱芜黑猪与大约克的肠黏膜上皮细胞与E.coliF18的黏附特性并无品种差异。但是养猪生产实践中,莱芜黑猪极少发生断奶仔猪水肿和腹泻病。所以除FUT I基因外,也许本地猪种有其他导致遗传抗性的突变或抗性基因。
王继英
,
郭建凤
,
孙守礼
,
郭立辉
,
蔺海潮
,
王诚
,
武英
. 莱芜黑猪a1岩藻糖转移酶基因(FUT I)不同基因型对大肠杆菌F18抵抗力研究[J]. 华北农学报, 2009
, 24(5)
: 64
-68
.
DOI: 10.7668/hbnxb.2009.05.014
On the basis of detect ion of FUT I genotypes of Laiwu Black Pigs and Yorkshire using PCR-RFLP,small intestinal epithelium cells of different genotypes,susceptive genotype and resistant genotype,were selected to test resistance and adhesion capability to the wild type enterotoxigenic Escherichia coli (ETEC F18).Because of several reasons,the pigs orally taken E.coli F18 did not catch the diseases,and it was impossible to judge their resistance to <>E.coli F18.The result dedicated that E.coli F18 could adhere to all the small intestinal epithelium cells of suscept ive genotype and not to the small intestinal epithelium cells of resistant genotype.There was not difference of adhesion capability in Laiwu Black Pigs and Yorkshire.But in the pig product ion,post-weaning diarrhea and edema disease seldom happened in Laiwu Black Pigs.Therefore,except of FUT I gene,perhaps there are other mutation and resistant gene of E.coli F18 in Laiwu Black Pigs and other indigenous pig breeds of China.
[1] V(o)geli P,Bertschinger H U,Stamm M,et al.Genes specifying receptors for F18 fimbriated Escherichia coli,causing oedema disease and postweaning diarrhoea in pigs,map to Chromosome 6[J].Anim Genet,1996,27(5):321-328.
[2] Bertschinger H U,Bachmann M,Mettler C,et al.Adhesive fimbriae produced in vivo by Escherichia coli O139:K12(B):H1 associated with enterotoxaemia in pigs[J].Veterinary Microbiology,1990,25(2-3):267-281.
[3] 罗艳茹.仔猪E.coli F18黏附型与FUT I基因型相关性研究[D].兰州:甘肃农业大学,2007.
[4] 李玉华,李何君,邱小田,等.大肠杆菌K88的黏附性与仔猪初生重、断奶重和日增重的关系[J].中国畜牧杂志,2007,43(5):1-3.
[5] 吴圣龙,原志伟,鞠慧萍,等.苏太仔猪FUT I基因M307 位点多态性与F18 大肠杆菌抗病相关性的体外鉴定[J].中国预防兽医学报,2007,29(10):783-787.
[6] 王继英,姜运良,郭建凤,等.猪FUT I基因多态性及其与生长性能的关联性分析[J].华北农学报,2008,23(4):65-68.
[7] 晏学明,任军,郭源梅,等.猪a1岩藻糖转移酶基因(FUT I)在26个中外猪种中的遗传变异研究[J].遗传学报,2003,30(9):830-834.
[8] 晏学明,郭源梅,丁能水,等.不同品种猪a1岩藻糖转移酶基因遗传变异初析[J].中国畜牧杂志,2004,40(3):8-10.
[9] 施启顺,谢新民,柳小春,等.猪肠毒素大肠杆菌ETEC F18受体基因型检测报告[J].遗传,2002,24(6):656-658.
[10] 施启顺,黄生强,柳小春,等.不同猪种E.coli F18受体基因的多态性[J].遗传学报,2003,30(3):221-224.
[11] 张引红,周忠孝,曹果清.FUTI 基因多态性及其与产仔性状的关联性研究[J].遗传,2007,29(1):52-56.
[12] 孙鹏翔,吴圣龙,包文斌,等.苏太猪FUT I基因多态性及其与生产性能的关联分析[J].中国畜牧杂志,2007,43(5):4-6.
[13] 姜勋平,刘永刚,熊远著,等.猪FUT 1基因对肉质和胴体性状的影响[J].遗传,2005,27(4):566-570.
[14] 何小平,彭勇波,樊斌.猪α1岩藻糖转移酶基因多态性检测及与性状关联的研究[J].养猪,2006,26(3):26-28.
[15] Cutler S A,Lonergan S M,Cornick N,et al.Dietary Inclusion of colicin E1 is effective in preventing postweaning diarrhea caused by F18-positive Escherichia coli in pigs[J].Antimicrobial Agents and Chemotherary,2007,51:3830-3835.
[16] Madec F,Bridoux N,Bounaix S,et al.Experimental models of porcine post-weaning colibacillosis and their relationship to post-weaning diarrhoea and digestive disorders as encountered in the field[J].Vet Microbiol,2000,72:295-310.