利用PCR技术首次从新疆巴州牦牛基因组DNA中扩增获得了乳铁蛋白(Lactoferrin,LF)基因exon 2编码区，其中含有乳铁蛋白素(Lactoferricin,Lfcin)基因编码区序列；利用在线生物软件对Lfcin蛋白的结构进行预测。结果表明：克隆获得了含新疆巴州牦牛LF基因exon 2的DNA序列(GenBank：EU547256)，共778 bp，其中LF基因exon 2编码区长165 bp，Lfcin基因编码区长75 bp；序列分析显示，克隆获得的牦牛DNA序列与奶牛该序列存在11个碱基的变异；牦牛和奶牛的Lfcin蛋白质序列完全相同，各物种Lfcin蛋白具有较高的同源性；各物种的Lfcin蛋白进化树符合物种进化规律；同源建模预测的Lfcin 3D模型显示，Lfcin为一段由α-螺旋和β-折叠组成的“U”型结构。首次从新疆巴州牦牛品种中克隆获得Lfcin基因编码区全长序列并揭示了其分子特征，为牦牛Lfcin蛋白基因工程和蛋白质功能方面的研究奠定了重要基础。
裴杰
,
阎萍
,
冯瑞林
,
梁春年
,
郭宪
,
曾玉峰
,
包鹏甲
,
朱新书
,
褚敏
. 新疆巴州牦牛Lfcin基因的克隆与分子特征[J]. 华北农学报, 2009
, 24(5)
: 50
-54
.
DOI: 10.7668/hbnxb.2009.05.011
Using PCR technology,the exon 2 of Lactoferrin gene was obtained from genome of Xinjiang Bazhou yak,the product containin the coding region of the Lfcin gene;the structure of Lfcin was predicted by the online biological software.As a result,The DNA sequence that contains the LF gene second exon of Xinjiang Bazhou yak was cloned(GenBank:EU547256),constituted by 778 bp,Lfcin coding region that code 25 amino acids being 75 bp length;According sequence analyse,there are 11 different bases between cow and yak′s sequence;Yak and cow′s Lfcin gene code same protein sequence and there are high homology in different species;the evolutionary tree of Lfcin protein in all species complies with laws of evovution;homogenous 3D model of Lfcin obtained by predict shows that Lfcin protein presents ″U2shape″structure.The coding region of Lfcin gene in Xinjian Bazhou yak was cloned for the first time,and its molecular characteristics was shown.This work laid an essential foundation for researches of genetics engineering and protein function about Lfcin protein of yak.
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