以85个单片段代换系为材料,其受体亲本为广陆矮4号,供体亲本为日本晴。通过单因素方差分析和Dunnett’s多重比较,分析单片段代换系与受体亲本之间穗粒数的差异,对代换片段上的穗粒数QTL进行鉴定。以P≤0.001为阈值共检测到27个穗粒数QTLs,分布于除第10染色体外的其他11条染色体上。其中,2个QTLs的加性效应表现为减效作用,另外25个穗粒数QTLs的加性效应表现为增效作用。其加性效应值的变化范围为-37.5～96.9,加性效应百分率的变化范围为-29.07%～88.57%。为进一步发掘和利用新的水稻穗粒数QTL奠定基础。
朱金燕
,
杨梅
,
王中德
,
王军
,
周勇
,
杨杰
,
范方军
,
李文奇
,
梁国华
,
仲维功
. 利用染色体单片段代换系定位水稻穗粒数QTL[J]. 华北农学报, 2013
, 28(4)
: 23
-30
.
DOI: 10.3969/j.issn.1000-7091.2013.04.005
Grain number is one of the important components of rice yield which is a typical quantitative trait and can easily be influenced by genetic background and environmental factors．Single segment substitution lines (SSSLs) can effectively reduce the interference of the genetic background．We developed a set of genome- wide SSSLs carrying Nipponbare introgression segments in the background of Guangluai 4．Quantitative trail loci (QTL) analysis involved one- way ANOVA and Dunnett' s test for comparing SSSLs with the recurrent parent Guangluai 4 (P 0．001)．We detected 27 QTLs for grain number distributed on 11 rice chromosomes except chromosome 10，of which 2 had negative effect and the others positive effect．The additive effect of these QTLs ranged from－37．5 to 96．6，with additive effect percentages－29．07% to 88．57%．These results provide useful information for exploring and using new grain number related resources．
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