将口蹄疫病毒(FMDV)结构蛋白基因P1的完整cDNA序列插入原核表达性载体pET-28α(+)中，获得融合表达质粒pET-P1，转化E.coli.BL21(DE3)，经IPTG诱导，SDS-PAGE结果表明，pET-P1获得融合表达，Western Blot检测证实表达的融合蛋白具有免疫活性，表达产物主要以包涵体的形式存在，进一步采用纯化试剂盒纯化P1蛋白做为诊断抗原。
常惠芸
,
丛国正
,
独军政
,
邵军军
,
林彤
,
冯金瑞
,
刘萍
. C型口蹄疫病毒衣壳蛋白前体P1基因的原核表达及其生物活性的初步分析[J]. 华北农学报, 2009
, 24(5)
: 7
-10
.
DOI: 10.7668/hbnxb.2009.05.002
The complete gene encoding the structural protein of FMDV (P1) was subcloned into expression vector pET228α(+),resulting in the fusion expression plasmid pET2P1.After transformed into E.coli (DE3) and induced by IPTG,the results of SDS2PAGE showed that the fusion protein was expressed in high level.The molecular weight of the fusion protein was 85 kDa and the expressed products were significant at inclusion body.Western blotting was performed to confirm that the expressed fusion protein could specifically react with antiserum against FMDV.The fusion protein were further purified and vaccined antigen based on the purified protein was developed.
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